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Anti mouse cd73 mab clone ty 23

Manufactured by BioXCell
Sourced in United States

Anti-mouse CD73 mAb (clone TY/23) is a monoclonal antibody that binds to the mouse CD73 protein. CD73 is an enzyme involved in the extracellular conversion of adenosine monophosphate (AMP) to adenosine.

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4 protocols using anti mouse cd73 mab clone ty 23

1

Multicolor Flow Cytometry Immunophenotyping

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Anti-mouse CD73 mAb (clone TY/23) and Rat IgG2a isotype control (clone 2A3) were purchased from BioX-Cell (West Lebanon, NH, USA). Anti-mouse mAbs for flowcytometric analysis were used as described here. FITC-conjugated anti-CD8a (53–6.7), anti-CD11b (M1/70), and PE-conjugated anti-CD4 (GK1.5), anti-CD39 (Duha59), anti-CD45 (30-F11), anti-Ly-6G/Gr-1 (RB6-8C5), anti-IFN-γ (XMG1.2), and APC-conjugated anti-CD3 (17A2), anti-CD45 (30-F11), anti-CD73 (TY/11.8), and BV421 conjugated anti-CD4 (GK1.5), anti-CD45 (30-F11) and mouse recombinant Interleukin-2 (r-IL-2) were purchased from BioLegend (San Diego, CA, USA). FcR blocking reagent was obtained from Miltenyi Biotec GmBH (Bergisch Gladbach, Germany). 7-AAD (7-Aminoactinomycin D) and FVS780 were purchased from Thermo-Fisher Scientific (Waltham, MA, USA) and BD Biosciences (Franklin Lakes, NJ, USA), respectively.
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2

Characterization of Murine T Cell Subsets

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Anti-mouse CD73 mAb (clone TY/23) and Rat IgG2a isotype control (clone 2A3) were purchased from BioX-Cell (West Lebanon, NH, USA). Anti-mouse mAbs for owcytometric analysis were used as described here. FITC-conjugated anti-CD4 (GK1.5), anti-CD8α (53 - 6.7), anti-CD11b (M1/70), and PE-conjugated anti-CD39 (Duha59), anti-IFN-γ (XMG1.2), APC-conjugated anti-CD73 (TY/11.8), anti-CD3 (17A2), and FITC-conjugated anti-CD45 (30-F11), and mouse recombinant Interleukin-2 (r-IL-2) were purchased from BioLegend (San Diego, CA USA). FcR blocking reagent was obtained from Miltenyi Biotec GmBH (Bergisch Gladbach, Germany). 7AAD (7-Aminoactinomycin D) and FVS-780 were purchased from Thermo-Fisher Scienti c (Waltham, MA USA) and BD Biosciences (Franklin Lakes, NJ USA), respectively.
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3

Antibody-based Immune Cell Analysis

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Anti-mouse CD73 mAb (clone TY/23) and Rat IgG2a isotype control (clone 2A3) were purchased from BioX-Cell (West Lebanon, NH, USA). Anti-mouse mAbs for owcytometric analysis were used as described here. FITC-conjugated anti-CD4 (GK1.5), anti-CD8α (53-6.7), anti-CD11b (M1/70), and PE-conjugated anti-CD39 (Duha59), anti-IFN-g (XMG1.2), APC-conjugated anti-CD73 (TY/11.8), anti-CD3 (17A2), and FITCconjugated anti-CD45 (30-F11), and mouse recombinant Interleukin-2 (r-IL-2) were purchased from BioLegend (San Diego, CA USA). FcR blocking reagent was obtained from Miltenyi Biotec GmBH (Bergisch Gladbach, Germany). 7AAD (7-Aminoactinomycin D) and FVS-780 were purchased from Thermo-Fisher Scienti c (Waltham, MA USA) and BD Biosciences (Franklin Lakes, NJ USA), respectively.
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4

Antibody-based Immune Cell Analysis

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Anti-mouse CD73 mAb (clone TY/23) and Rat IgG2a isotype control (clone 2A3) were purchased from BioX-Cell (West Lebanon, NH, USA). Anti-mouse mAbs for owcytometric analysis were used as described here. FITC-conjugated anti-CD4 (GK1.5), anti-CD8α (53-6.7), anti-CD11b (M1/70), and PE-conjugated anti-CD39 (Duha59), anti-IFN-g (XMG1.2), APC-conjugated anti-CD73 (TY/11.8), anti-CD3 (17A2), and FITCconjugated anti-CD45 (30-F11), and mouse recombinant Interleukin-2 (r-IL-2) were purchased from BioLegend (San Diego, CA USA). FcR blocking reagent was obtained from Miltenyi Biotec GmBH (Bergisch Gladbach, Germany). 7AAD (7-Aminoactinomycin D) and FVS-780 were purchased from Thermo-Fisher Scienti c (Waltham, MA USA) and BD Biosciences (Franklin Lakes, NJ USA), respectively.
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