Nb200 540b

Manufactured by Novus Biologicals

The NB200-540B is a laboratory instrument designed for electrophoresis separation and visualization of biomolecules. The core function of this product is to facilitate the separation and analysis of DNA, RNA, or protein samples.

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Lab products found in correlation

1 step ultra tmb elisa substrate solution, by Thermo Fisher Scientific (2 mentions) High binding elisa plates, by Thermo Fisher Scientific (2 mentions) Streptavidin hrp, by Mabtech (2 mentions) Serum gel, by Sarstedt (1 mentions)

2 protocols using nb200 540b

Quantifying Complement Binding via ELISA

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High binding ELISA plates (07-200-37, Fisher Scientific) were coated with 1 μg/mL of labeled eOD-60mer₄₀ and blocked with 2% BSA in PBS. Serum was collected from naïve mice using collection tube with Serum Gel (41.1500.005, Sarstedt), diluted in PBS (3% v/v), and incubated in ELISA plates for 1 hour at 37°C. Complement binding was detected by biotinylated anti-C3 antibody (NB200-540B, Novus Biologicals) followed by streptavidin-HRP (3310-9-1000, Mabtech AB). The plates were developed with 1-Step Ultra TMB-ELISA Substrate Solution (34028, Thermo Fisher Scientific) and the reaction was stopped by adding with 2 N sulfuric acid (BDH7500-1).

Tavernier J., Tuypens T., Verhee A., Plaetinck G., Devos R., Van der Heyden J., Guisez Y, & Oefner C. (1995). Identification of receptor-binding domains on human interleukin 5 and design of an interleukin 5-derived receptor antagonist.. Proceedings of the National Academy of Sciences of the United States of America, 92(11), 5194-5198.

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Complement Binding ELISA Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

High binding ELISA plates (07-200-37, Fisher Scientific) were coated with 1 µg/mL of labeled and blocked with 2% BSA in PBS. Serum was collected from naïve mice (41.1500.005, Sarstedt), diluted in PBS (3% v/v), and incubated in ELISA plates for 1 hour at 37°C. Complement binding was detected by biotinylated anti-C3 antibody (NB200-540B, Novus Biologicals) followed by streptavidin-HRP (3310-9-1000, Mabtech AB). The plates were developed with 1-Step Ultra TMB-ELISA Substrate Solution (34028, Thermo Fisher Scientific) and the reaction was stopped by adding with 2 N sulfuric acid (BDH7500-1).

Aung A., Cui A., Amini A.P., Bukenya M., Lee H., Cottrell C.A., Silva M., Kirkpatrick J.D., Gregory J.R., Amlashi P., Remba T., Froehle L.M., Xiao S., Abraham W., Adams J., Suh H., Huyett P., Kwon D.S., Hacohen N., Schief W.R., Bhatia S.N., & Irvine D.J. (2021). Spatially regulated protease activity in lymph nodes renders B cell follicles a sanctuary for retention of intact antigens.

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