Coomassie blue g 250

The levels of serum albumin, total protein, serum creatinine and blood urea nitrogen, TAG, total cholesterol, LDL, HDL, fasting blood glucose, insulin and homoeostasis model assessment of insulin resistance (HOMA-IR) were determined by automatic clinical chemistry analyzer (Modular Corporation). Coomassie Blue G-250 (Nanjing Jiancheng Bioengineering Institute) was used to detect 24-h proteinuria in the rats. The levels of malondialdehyde (MDA), protein carbonylation, GSH peroxidase (GSH-PX) and superoxide dismutase (SOD) in serum were detected using oxidative stress detection kits (Nanjing Jiancheng Bioengineering Institute). Levels of AngII in the renal cortex were detected using RIA kits (provided by Shanghai Chinese Traditional Medical University). ELISA kits (R&D Systems) were used to measure the levels of AngII in serum and supernatant of cultured cells. All of the samples for measuring AngII were pre-treated by passing them through a Centricon-10 column with a cut-off of >10 000 Da (Amicon; EMD Millipore). The amount of fibronectin (FN) and TGF-β1 released in the cell culture medium was also detected using ELISA kits.