The particle size and morphology of silica-coated magnetic nanoparticles were determined by transmission electronic microscopy (TEM) using a JEM-4010 (JEOL; Tokyo, Japan) at an accelerating voltage of 400 kV. The magnetic nanoparticles and silica-coated magnetic nanoparticles were characterized by powder X-ray diffraction (XRD), using a Rigaku D/MAX-2500 diffractometer (Rigaku; Tokyo Japan) using filtered Cu Kα radiation, and the data were collected for 2θ of 20.0° to 80.0°. The magnetization of silica-coated magnetic nanoparticles at room temperature up to 10 kOe was measured by vibrating sample magnetometer (VSM) using a VSM 4179 (Oxford Instruments; Oxfordshire, UK). Fourier transform infrared (FT-IR) spectroscopy was used to identify the functionalized MNPs@SiO2. The FT-IR spectra were recorded on a V-460 (JASCO; Easton, MD, USA) FT-IR spectrometer using KBr pellets. Spectra were obtained at a resolution of 4 cm-1, and the wavenumber range from 4,000 to 650 cm-1. The fluorescence intensity and absorbance of the samples were measured using microplate reader Infinite M200 (Tecan Ltd). The fluorescence was measured five times for each sample with a 20-μs integration time.
V 460
Manufactured by Jasco
Sourced in United States
The V-460 is a versatile laboratory equipment that can be used for a variety of scientific applications. It is designed to perform basic measurements and analysis tasks. The core function of the V-460 is to provide accurate and reliable data for research and experimentation purposes.
Automatically generated - may contain errors
2 protocols using v 460
1
Characterization of Silica-Coated Magnetic Nanoparticles
2
Spectrophotometric Laccase Activity Assay
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Laccase activity was routinely measured with a spectrophotometer (V460, Jasco, Easton, MD, USA) using ABTS (ε 420nm = 36 mM -1 cm -1 ) as a substrate: assay mixtures (1 ml) contained 1 mM ABTS, 10 μl of purified protein or 100 μl of crude extract, 50 μM CuSO 4 in a multi-component buffer at pH 4.0 (10 mM Trizma base, 15 mM sodium carbonate, 15 mM phosphoric acid and 250 mM potassium chloride) (Harris et al. 2001) . Laccase from Trametes versicolor (Sigma-Aldrich) was used as positive control. All assays were performed in triplicate. The oxidation of the ABTS was followed as the change in absorbance at 420 nm and 25 °C for 5 min. One activity unit (U) was defined as the amount of enzyme required for oxidizing 1 μmol of ABTS per min.
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