Six color flow cytometry was performed using CD45 and side scatter gating. While the complete panel of antibodies employed varied depending on the available number of cells, B-cell clonality was evaluated in all cases using the following six color combination: CD5-perdinin chlorophyll protein complex-Cy5.5 (PerCP-Cy5.5), CD19-allophycocyanin (APC), CD20-APC-Cy7, CD45-phycoerythrin (PE)-Cy7, and κ-fluorescein isothiocyanate and λ-PE immunoglobulin light chains (Becton Dickenson, San Jose, CA). Analysis was completed using an FACSCanto flow cytometer (Becton Dickinson). Flow cytometry data were analyzed using FCS Express (v3.0, De Novo Software, Los Angeles, CA).
Cd45 phycoerythrin pe cy7
Manufactured by BD
CD45-phycoerythrin (PE)-Cy7 is a fluorochrome-conjugated antibody that binds to the CD45 antigen, which is expressed on the surface of various hematopoietic cells. The PE-Cy7 fluorochrome combination provides a bright signal and is commonly used in flow cytometry applications.
Automatically generated - may contain errors
2 protocols using cd45 phycoerythrin pe cy7
1
Six-Color Flow Cytometry for B-Cell Clonality
2
Six-Color Flow Cytometry for B-Cell Clonality
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