Sorafenib n oxide

Sorafenib and Sorafenib N-oxide were purchased from Sigma-Aldrich (St. Louis, MO, USA). The ultra-performance liquid chromatography (UPLC)@BEH C18 column (2.1 mm × 50 mm, 1.7 μm) was purchased from Millipore (Bedford, MA, USA). NADPH was obtained from Promega (Madison, WI, USA). Recombinant human cDNA encoding wild-type (CYP3A5*1) gene or each of six variant genes (CYP3A5*2, CYP3A5*3A, CYP3A5*3C, CYP3A5*4, CYP3A5*5, CY-P3A5*6, and CYP3A5*7) was purchased from Wuhan Bafeier Biological (Wuhan, China). Sorafenib N-oxide as internal standard was purchased from Sigma-Aldrich (St Louis, MO, USA). HPLC-grade solvents were obtained from Fisher Scientific (Pittsburgh, PA, USA), and analytical grade reagents were obtained from Merck Chemicals (Darmstadt, Germany). Rabbit anti-human CYP3A5 IgG primary antibody was obtained from Sigma-Aldrich, and the secondary goat anti-rabbit IgG-HRP-conjugated antibody was purchased from Shanghai Gu Ge Biology (Shanghai, China).