Glutamine synthetase (GS), methionine sulfoximine (MSX), Chinese hamster ovary K1 (CHO-K1) cells, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin (P/S), insulin, and pentobarbital sodium were supplied by Sigma-Aldrich (St. Louis, MO, United States). Biotin-conjugated monoclonal antibodies for ELISA were purchased, including osteoprotegerin (OPG) ab255723, ADPN ab108784 (Abcam, Cambridge, United Kingdom). Antibodies for Western blot were GAPDH ab8245, AdipoR1 ab70362, BMP-2 ab14933 (Abcam, Cambridge, United Kingdom), and ALP sc-271431 and Osteocalcin (OCN) sc-376726 (Santa Cruz, CA, United States). Antibodies used for immunofluorescent staining were BMP-2 ab6285 (Abcam, Cambridge, United Kingdom); ALP sc-271431, OCN sc-390877 (Santa Cruz, CA, United States); and AdipoR1 BM4566 (Boster Bio, CA, United States).
Gong Y., Wang Y., Zhang Y., Wang L., Wan L., Zu Y., Li C., Wang X, & Cui Z.K. (2021). Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration. Frontiers in Cell and Developmental Biology, 9, 762335.
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