InnovaCoat® GOLD 40 nm Streptavidin gold nanoparticles (streptAv-AuNPs) were purchased from Innova Biosciences (Cambridge, UK). Dynabeads™ M-280 Tosylactivated magnetic particles (2.8 μm, 30 mg mL−1, 2 × 109 MP mL−1) were provided by Invitrogen Dynal AS (Oslo, Norway).
Polyclonal antibody to anti-LAM from Mycobacterium (MBS315001) was from MyBioSource (San Diego, CA, USA). All buffer solutions were prepared with Milli-Q water and all other reagents were in analytical reagent grade (supplied from Sigma). The composition of these solutions were: borate buffer (0.1 mol L−1 boric acid, pH 8.5); conjugate diluting buffer (2 mmol L−1 borate pH 7, 10% w/v sucrose); running buffer (0.01 mol L−1 phosphate buffer pH 7.4, 1% BSA, 0.05% Tween 20); ammonium sulfate buffer ((NH4)2SO4 3 mol L−1 in borate buffer); blocking buffer/PBS 0.5% BSA (10 mmol L−1 phosphate, 0.5% w/v BSA, pH 7.4); washing buffer/PBS 0.1% BSA (10 mmol L−1 phosphate, 0.1% w/v BSA, pH 7.4); sample pad buffer (0.01 mol L−1 phosphate buffer pH 7.4, 1% BSA, 0.05% Tween 20).
Polyclonal antibody to anti-LAM from Mycobacterium (MBS315001) was from MyBioSource (San Diego, CA, USA). All buffer solutions were prepared with Milli-Q water and all other reagents were in analytical reagent grade (supplied from Sigma). The composition of these solutions were: borate buffer (0.1 mol L−1 boric acid, pH 8.5); conjugate diluting buffer (2 mmol L−1 borate pH 7, 10% w/v sucrose); running buffer (0.01 mol L−1 phosphate buffer pH 7.4, 1% BSA, 0.05% Tween 20); ammonium sulfate buffer ((NH4)2SO4 3 mol L−1 in borate buffer); blocking buffer/PBS 0.5% BSA (10 mmol L−1 phosphate, 0.5% w/v BSA, pH 7.4); washing buffer/PBS 0.1% BSA (10 mmol L−1 phosphate, 0.1% w/v BSA, pH 7.4); sample pad buffer (0.01 mol L−1 phosphate buffer pH 7.4, 1% BSA, 0.05% Tween 20).