PBMCs from patients with SLE or RA and HC were collected and analyzed immediately for the molecular phenotypes of lymphocytes. The antibodies used for the surface marker analysis include anti-human CD3-ECD (Beckman Coulter, USA), CD4-Percp-Cy5.5, CD8-PE-Cy7, PD1-FITC, TIGIT-APC, ICOS-PE, TIM3-BV421, CD19-V500, CD3-FITC, CD8-V500, CD25-APC, HLA-DR- PE-Cy7, CD69-V450, and CD127-PE (BD Biosciences, USA). Briefly, 50 μl of cells was incubated with appropriate antibodies on ice in the dark for 30 min, followed by washing in PBS. All the samples were analyzed with a Navios flow cytometer (Beckman Coulter) and Kaluza analysis software (Beckman Coulter).
Cd69 v450
Manufactured by BD
Sourced in United States
CD69-V450 is a fluorescent antibody used for the detection and quantification of the CD69 antigen, which is a cell surface glycoprotein expressed on activated T cells, B cells, and natural killer cells. The antibody is conjugated with the V450 fluorescent dye, which emits light in the violet region of the visible spectrum. This product is intended for use in flow cytometry applications to identify and analyze immune cell activation.
Automatically generated - may contain errors
Lab products found in correlation
Hla dr pe cy7, by BD (1 mentions)
Cd8 v500, by BD (1 mentions)
Cd4 percp cy5, by Beckman Coulter (1 mentions)
Tim3 bv421, by BD (1 mentions)
Anti human cd3 ecd, by Beckman Coulter (1 mentions)
Cd127 pe, by BD (1 mentions)
Pd1 fitc, by BD (1 mentions)
Cd19 v500, by BD (1 mentions)
Icos pe, by BD (1 mentions)
Kaluza analysis software, by Beckman Coulter (1 mentions)
Navios flow cytometer, by Beckman Coulter (1 mentions)
Cd3 fitc, by BD (1 mentions)
Cd8 pe cy7, by BD (1 mentions)
Cd25 apc, by BD (1 mentions)
Cd56 pe, by BioLegend (1 mentions)
Cd25 pe cy7, by BD (1 mentions)
Cd16 apc cy7, by BD (1 mentions)
Fixable viability stain 510, by BD (1 mentions)
Granzyme b af647, by BD (1 mentions)
Pbs 2 bsa, by Merck Group (1 mentions)
2 protocols using cd69 v450
1
Comprehensive Immune Profiling in SLE and RA
2
Flow cytometric analysis of NK cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used for flow cytometry analysis: CD45 FITC (BD Biosciences, clone H130), CD56 PE (Biolegend, clone HCD56), CD3 BV605 (BD Biosciences, clone SK7), CD69 V450 (BD Biosciences, clone FN50), CD16 APC-Cy7 (BD Biosciences, clone 3G8), CD25 PE-Cy7 (BD Biosciences, clone M-A251), Granzyme B AF647 (BD Biosciences, clone GB11) and EGFR BV421 (BD Bioscience, clone EGFR.1). Cells were incubated with antibodies diluted in staining buffer (PBS +2% BSA, both Sigma Aldrich) for 25 min at 4°C in the dark. Cells were washed twice and stained with Fixable Viability Stain 510 (BD Bioscience) for 15 min at room temperature for live/dead cell discrimination. After two washes, NK cells were analyzed by flow cytometry (BD FACSCanto IVD 10, standard 3-lasers, 10 colors, BD Biosciences). Unstained control sample was used to set up PMT voltages. Single stain controls with BD CompBeads compensation particles (BD Biosciences) were used to optimize fluorescence compensation settings. Data analysis was performed with FlowJo Software v10.7.1 (FlowJ0, Becton, Dickinson and Company).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!