Tissues were fixed overnight in 10% formalin, embedded in paraffin, and cut into 5-μm-thick sections for histological examination. The specimens were then deparaffinized for Masson trichrome (MT) staining. For immunohistochemistry, anti-αSMA, anti-CD45, anti-F4/80 and anti-CD3 antibodies were obtained from BD Biosciences (NJ). Nuclei were counterstained using DAPI (Life Technologies, CA). Fluorescence was observed using a fluorescence microscope equipped with the appropriate filter sets (BZ-900, KEYENCE, Osaka, Japan). Areas of interest were quantified using a BZ-H3C module (KEYENCE).
Bz h3c module
Manufactured by Keyence
The BZ-H3C module is a compact and versatile imaging device designed for microscopy applications. It provides high-quality image capture and analysis capabilities, enabling users to observe and document samples with precision. The module's core function is to serve as an imaging solution for various scientific and research purposes.
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Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Anti cd45, by BD (1 mentions)
Anti cd3, by BD (1 mentions)
Anti f4 80, by BD (1 mentions)
Anti α sma, by BD (1 mentions)
Bz 900, by Keyence (1 mentions)
Bz x710 microscope, by Keyence (1 mentions)
Rat anti mouse f4 80 antibody, by Bio-Rad (1 mentions)
2 protocols using bz h3c module
1
Histological Analysis of Tissue Samples
2
Histological Analysis of Tissue Inflammation
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Tissues were fixed overnight in 10% formalin, embedded in paraffin, and cut into 5-μm-thick sections for histological examination. The specimens were then deparaffinized for Berlin blue staining. For immunohistochemical analysis, rat anti-mouse F4/80 antibody (BIO-RAD, Hercules, CA) and rat anti-mouse 4-hydroxy-2-nonenal (4HNE) (NOF Corporation, Tokyo, Japan) were used. DAPI (Thermo Fisher Scientific, MA) was used to stain the nuclei. Sections were observed using a KEYENCE model BZ-X710 microscope (Osaka, Japan). The area of interest was quantified using the BZ-H3C module with the BZ-X710 microscope (KEYENCE).
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