5-tricosyl-resorcinol, 5-heptadecylresorcinol (10 mg powder), 2,5-dihydroxybenzoic
acid (DHB), and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich
(Steinheim). Gelatin used for embedding was obtained from VWR International
(Darmstadt, Germany). Glass microscope slides (ground edges, super
frost) were obtained from R. Langenbrinck (Emmendingen, Germany).
LC–MS-grade methanol, ethyl acetate, and 2-propanol were purchased
from Scharlab Italia Srl (Milan, Italy); bidistilled water was obtained
using a Milli-Q system (Millipore, Bedford, MA, USA). MS-grade ammonium
formate and formic acid from Fisher Chemical (Thermo Fisher Scientific,
Inc., San Jose, CA, USA) were also used.