Dioleoyl phosphatidylglycerol

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), dioleoyl phosphatidylcholine (DOPC), dioleoyl phosphatidylglycerol (DOPG), Sphingomyelin (SM), N-(7-nitro-benz-2-oxa-1,3-diazol-4-yl) phosphatidylethanolamine (NBD-PE) and N-(Lissamine-rhodamine-B-sulfonyl) phosphatidylethanolamine (Rho-PE) were purchased from Avanti Polar Lipids (Birmingham, AL, USA), while cholesterol (CHOL), Triton 100X and 4-chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD-Cl) were purchased from Sigma (St. Louis, MO, USA). DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate) was purchased from Invitrogen (Carlsbad, CA, USA), Sudan Black B from Sigma-Aldrich (St. Louis, MO, USA). Picric acid, acetic acid, formaldehyde and paraffin wax were from Carlo Erba (Milan, IT).

Dihexanoylphosphatidylglycerol (DHPG), dipalmitoylphosphatidylglycerol (DPPG), distearoylphosphatidylglycerol (DSPG), palmitoyl-oleoylphosphatidylglycerol (POPG), dioleoylphosphatidylglycerol (DOPG), palmitoyl-arachidonoylphosphatidylglycerol (PAPG), palmitoyl-linoleoylphosphatidylglycerol (PLPG), dilinoleoylphosphatidylglycerol (DLPG), soy-derived PG (soy PG), egg-derived PG, and dilinoleoylphosphatidylpropanol (DLPP) were all obtained from Avanti Polar Lipids, Inc. (Alabaster, AL). The composition of egg PG is provided in the Introduction. Soy PG is composed of 16∶0 (17%), 18∶0 (6%), 18∶1 (13%), 18∶2 (59%), and 18∶3 (5%) (Avanti Polar Lipids website). Calcium-free minimal essential medium and antibiotics were obtained from Biologos, Inc. (Maperville, Illinois). Bovine pituitary extract and epidermal growth factor were purchased from Life Technologies, Inc. (Grand Island, New York). ITS+(6.25 µg insulin per mL, 6.25 µg transferrin per mL, 6.25 ng selenous acid per mL, 5.35 mg linoleic acid per mL, and 0.125% bovine serum albumin) was supplied by Collaborative Biomedical Products (Bedford, Massachusetts).