Heat inactivated fbs

Manufactured by Cytiva

Sourced in Moldova, Republic of

Heat-inactivated FBS is a supplementary component for cell culture media. It provides essential nutrients and growth factors to support the growth and maintenance of various cell lines in vitro.

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Lab products found in correlation

Rpmi 1640, by Fujifilm (1 mentions) Phenol red, by Fujifilm (1 mentions) Ht 29, by Fujifilm (1 mentions) Dld 1, by Fujifilm (1 mentions) 0.4 m transwell inserts, by Corning (1 mentions) Hepes buffered rpmi 1640, by Lonza (1 mentions)

2 protocols using heat inactivated fbs

Culturing Luciferase-Expressing CRC Cell Lines

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Human CRC cell lines stably expressing luciferase (DLD-1 clone #1-Luc: JCRB1382, RRID: CVCL J250 and HT-29-Luc: JCRB1383, RRID:CVCL J256) were obtained from the Japanese Cancer Research Resources Bank (Suita City, Osaka, Japan). Human CRC cell lines (DLD-1, RRID: CVCL 0248 and HT-29, RRID: CVCL 0320) were obtained from Taiho Pharmaceutical (Shibuya-ku, Tokyo, Japan). DLD-1 clone #1-Luc, DLD-1, and HT-29 cells were cultured in RPMI 1640 and Phenol Red (Fujifilm Wako, Chuo-ku, Osaka, Japan), and HT-29-Luc cells were cultured in McCoy’s 5A medium. All of the media were supplemented with 10% (v/v) heat-inactivated FBS (Hyclone Laboratories, Logan, UT) and incubated at 37°C and 5% CO₂.

Arima J., Taniguchi K., Sugito N., Heishima K., Tokumaru Y., Inomata Y., Komura K., Tanaka T., Shibata M.A., Lee S.W, & Akao Y. (2023). Antitumor effects of chemically modified miR-143 lipoplexes in a mouse model of pelvic colorectal cancer via myristoylated alanine-rich C kinase substrate downregulation. Molecular Therapy. Nucleic Acids, 34, 102079.

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Assaying Neutrophil-Helicobacter pylori Interactions

Cited 1 time

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Helicobacter pylori strains NCTC11637 (hereafter 11637) and 60190 were grown under microaerophilic conditions, washed, and quantified by measurement of the absorbance at 600 nm (15 (link), 17 (link)). Neutrophils were diluted in Hepes-buffered RPMI-1640 (Lonza, Walkersville, MD) containing 10% heat-inactivated FBS (HyClone Laboratories, Pittsburgh, PA) to 1×10⁶ cells/ml. H. pylori were added at a ratio of 5 bacteria/PMN, and cell suspensions were incubated at 37°C under microaerophilic conditions for up to 48 h. Some experiments used 0.4 µm Transwell inserts (Corning, Oneonta, NY), or included neutrophils that were pretreated with 5 µg/ml actinomycin D or 10 µg/ml cycloheximide, or H. pylori that were pretreated with 50 µg/ml chloramphenicol for 30 min, and drugs were maintained in the medium for the duration of the experiment.

Whitmore L.C., Weems M.N, & Allen L.A. (2017). Cutting Edge: Helicobacter pylori Induces Nuclear Hypersegmentation and Subtype Differentiation of Human Neutrophils In Vitro. The Journal of Immunology Author Choice, 198(5), 1793-1797.

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