HLMs and RLMs all were purchased from iPhase Pharmaceutical Services Co., Ltd. (Beijing, China). Metabolic probe substrates and their metabolites including midazolam, 1-hydroxymidazolam, bupropion, hydroxy bupropion, amodiaquine, N-desethyl amodiaquine, losartan, E-3174, mephenytoin, 4-hydroxy mephenytoin, dextromethorphan, and dextrorphan were purchased from Shanghai Canspec Scientific Instruments Co., Ltd. (Shanghai, China). The internal standard diazepam and vortioxetine were purchased from Beijing Sunflower Technology Development Co., Ltd. (Beijing, China). The reduced nicotinamide adenine dinucleotide phosphate (NADPH) used in the incubation system was purchased from Roche Pharmaceutical Ltd. (Basel, Switzerland). Methanol and acetonitrile used for UPLC-MS/MS were purchased from Merck (Darmstadt, Germany). Ultrapure water was obtained from Millipore (Bedford, MA, United States) by using a Milli-Q A10 purification system.
Reduced nicotinamide adenine dinucleotide phosphate nadph
Manufactured by Roche
Sourced in Switzerland
Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is a coenzyme involved in numerous cellular processes. It serves as an electron carrier, participating in various redox reactions within the cell. NADPH plays a vital role in biosynthesis, antioxidant defense, and energy metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
Ultrapure water, by Merck Group (1 mentions)
Milli q reagent system, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (1 mentions)
Triphenyl phosphate, by Merck Group (1 mentions)
Diethyl maleate, by Merck Group (1 mentions)
Phenylmethanesulfonyl fluoride, by Merck Group (1 mentions)
Piperonyl butoxide, by Merck Group (1 mentions)
Protein assay dye reagent, by Bio-Rad (1 mentions)
1 chloro 2 4 dinitrobenzene, by Merck Group (1 mentions)
Eserine, by Merck Group (1 mentions)
Fast blue b salt, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid edta, by Sinopharm (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Reduced glutathione, by Merck Group (1 mentions)
Metaflumizone, by BASF (1 mentions)
Spinosad, by Dow (1 mentions)
Spinetoram, by Dow (1 mentions)
7 protocols using reduced nicotinamide adenine dinucleotide phosphate nadph
1
UPLC-MS/MS Metabolic Profiling Assay
2
Loperamide and DLOP Metabolism Study
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Loperamide, DLOP and midazolam (used as internal standard) were purchased from Shanghai Canspec Scientific & Technology Co., Ltd. (Shanghai, China) Acetonitrile and methanol of analytical grade were bought from Merck (Darmstadt, Germany); formic acid was from Sigma-Aldrich Co. (St. Louis, MO, USA). Ultra-pure water was produced with a Milli-Q reagent system (Millipore, Bedford, MA, USA). The reduced nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Roche Pharmaceutical Ltd (Basel, Switzerland). Recombinant human CYP3A4 enzymes and purified cytochrome b5 were kind gifts from Beijing Hospital (Beijing, China).24 (link)–26 (link)
3
Insecticide Compounds Sourcing and Characterization
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Metaflumizone (240 g/L SC) was obtained from the BASF Chemical Co., Ltd. (Shanghai, China). Indoxacarb (95%), abamectin (95%), beta-cypermethrin (96.1%), chlorfluazuron (95%), chlorfenapyr (95%), diafenthiuron (98%), and chlorantraniliprole (95%) were purchased from Hubei Kangbaotai Fine-Chemicals Co., Ltd. (Wuhan, China). Spinetoram (60 g/L SC) and spinosad (25 g/L SC) were purchased from the Dow AgroSciences Co., Ltd. (Shanghai, China). BT WG-001 (16000 IU/mg) was supplied by the Hubei Biopesticide Engineering Research Center. The following were obtained from the Sigma Chemical Co., Ltd. (St. Louis, MO, USA): triphenyl phosphate (TPP, reagent grade); diethyl maleate (DEM, reagent grade); piperonyl butoxide (PBO, reagent grade); 1-chloro-2,4-dinitrobenzene (CDNB); reduced glutathione (GSH); fast blue B salt; sodium-dodecyl sulphate (SDS); dithiothreitol (DTT); eserine; phenylmethanesulfonyl fluoride (PMSF). Alpha-naphthol acetate (α-NA) and ethylene diamine tetra-acetic acid (EDTA) were purchased from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Reduced nicotinamide adenine dinucleotide phosphate (NADPH) was obtained from Roche Molecular Systems, Inc. (San Francisco, CA, USA). The protein-assay dye reagent was provided by Bio-Rad Laboratories, Inc. (Shanghai, China).
4
Quantitative Analysis of Drug Compounds
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Rivaroxaban (purity >99%) and diazepam (purity >98%) were both purchased from J&K Scientific Ltd. (Beijing, China) (Figure 1 ). Rivaroxaban metabolite M2 (Lot No. 3530-047A1) was purchased from TLC Pharmaceutical Standards Ltd. (Newmarket, Ontario). Ticagrelor (purity > 98%) was purchased from Aladdin (Aladdin, Shanghai, China). Reduced nicotinamide adenine dinucleotide phosphate (NADPH) was obtained from Roche Pharmaceuticals Ltd. (Basel, Switzerland). Acetonitrile and methanol were obtained from Fisher Scientific Co. (Fair Lawn, NJ, USA). Formic acid was got from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was obtained from a Milli-Q water purification system (Millipore, Billerica, MA, USA). All other chemicals were of analytical grade or better.
5
In Vitro Metabolism of Flumatinib
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Flumatinib (Figure 1A ), and M1 (the main metabolite of flumatinib, Figure 1B ) were provided kindly by Jiangsu Hansoh Pharmaceutical Group Co. Ltd. (Lianyungang, China). Isavuconazole (Figure 1C ), dasatinib (used as internal standard, IS, Figure 1D ) and other drugs were purchased from Shanghai Chuangsai Technology Co., Ltd. (Shanghai, China). HLM, RLM and rCYP3A4 were obtained from iPhase Pharmaceutical Services Co., Ltd. (Beijing, China). Reduced nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Roche Pharmaceutical Ltd. (Basel, Switzerland). All other unmentioned chemicals and solvents are available in analytical grade.
6
Characterization of Drug Metabolism Enzymes
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Osimertinib and AZ5104 were purchased from Beijing Sunflower and Technology Development Co., Ltd. (Beijing, China). Omeprazole, lansoprazole, and rabeprazole were purchased from Shanghai Canspec Scientific Instruments Co., Ltd. (Shanghai, China). Sorafenib was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Pooled RLMs and HLMs were from Corning Life Sciences Co., Ltd. Recombinant human CYP3A4 and cytochrome b5 were prepared by our group as indicated previously (Zhou et al., 2019 (link)). Reduced nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Roche Pharmaceutical Ltd. (Basel, Switzerland). All other chemicals and solvents not mentioned were of analytical grade. The information on 114 drugs is presented in Supplementary Table S1 .
7
Human Liver Microsome Metabolism Assay
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Lasiocarpine (purity > 97%) was purchased from Phytolab (Vestenbergsgreuth, Germany). Dehydromonocrotaline (purity ≥ 90%) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Potassium dihydrogen phosphate, trifluoroacetic acid (TFA) and formic acid were purchased from VWR International (Darmstadt, Germany). Phosphate buffered saline (PBS) was purchased from Gibco (Paisley, Scotland, UK). Phosphoric acid was obtained from Merck (Darmstadt, Germany). Reduced nicotinamide adenine dinucleotide phosphate (NADPH) was obtained from Roche Diagnostics (Mannheim, Germany). Acetonitrile (UPLC/MS grade) was obtained from Biosolve BV (Valkenswaard, The Netherlands). Glutathione (GSH), N,Ndimethylformamide (DMF) and dimethylsulfoxide (DMSO) were purchased from Sigma-Aldrich (Steinheim, Germany). Twenty-five individual male human liver microsomes of Caucasian origin were purchased from XenoTech (Lenexa, USA). Twenty-five individual male human liver microsomes of Chinese origin were purchased from PrimeTox (Wuhan, China). The characteristics of the human liver microsome donors are presented in Table S1 in the supplementary materials 1.
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