Reprogrammable MEFs grown in mESC media were dox-treated for 5 days, then harvested and stained for various surface markers: Anti-mouse CD326 EPCAM (PE), anti-mouse SSEA1 APC-647 (Biolegend) and anti-mouse CD90 THY1.2 eFluor 450 (eBiosciences). THY1+/SSEA1-/EPCAM-/OCT4-GFP-cells (“refractory cells”) were sorted in equal numbers onto gelatin-coated 6-well plates containing mESC media containing doxycycline, doxycycline+ascorbic acid, doxycycline+GSK3i or doxycycline+AGi. Media and small molecules were replaced every other day, for a total of 7 days, after which dox and AGi were withdrawn and replaced with fresh mESC media. Dox-independent iPSCs were scored at least three days later by alkaline phosphatase staining kit (Vector Labs SK-5100).
Anti mouse cd326 epcam
Manufactured by BioLegend
Anti-mouse CD326 (EPCAM) is a laboratory reagent used to detect and quantify the expression of the CD326 (also known as EPCAM) cell surface marker on mouse cells. CD326 is a transmembrane glycoprotein involved in epithelial cell adhesion.
Automatically generated - may contain errors
2 protocols using anti mouse cd326 epcam
1
Reprogramming Refractory MEFs to iPSCs
2
Reprogramming Refractory MEFs to iPSCs
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Reprogrammable MEFs grown in mESC media were dox-treated for 5 days, then harvested and stained for various surface markers: Anti-mouse CD326 EPCAM (PE), anti-mouse SSEA1 APC-647 (Biolegend) and anti-mouse CD90 THY1.2 eFluor 450 (eBiosciences). THY1+/SSEA1-/EPCAM-/OCT4-GFP-cells (“refractory cells”) were sorted in equal numbers onto gelatin-coated 6-well plates containing mESC media containing doxycycline, doxycycline+ascorbic acid, doxycycline+GSK3i or doxycycline+AGi. Media and small molecules were replaced every other day, for a total of 7 days, after which dox and AGi were withdrawn and replaced with fresh mESC media. Dox-independent iPSCs were scored at least three days later by alkaline phosphatase staining kit (Vector Labs SK-5100).
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