Cpg odn

Manufactured by Thermo Fisher Scientific

Sourced in United States

CpG-ODN is a synthetic oligodeoxynucleotide containing unmethylated CpG motifs. It functions as an agonist for Toll-like receptor 9 (TLR9), which is involved in the recognition of pathogenic DNA and the subsequent activation of the innate immune response.

Automatically generated - may contain errors

Lab products found in correlation

24 well insert, by Corning (1 mentions) Phytohemagglutinin (pha), by Merck Group (1 mentions) Anti cd69 pe, by BD (1 mentions) Anti human igm, by Jackson ImmunoResearch (1 mentions) Hmgb1, by R&D Systems (1 mentions) Primary and secondary antibodies, by Cell Signaling Technology (1 mentions) Tnf α, by R&D Systems (1 mentions) Il 1α, by R&D Systems (1 mentions) Flagellin, by Thermo Fisher Scientific (1 mentions) Tlr reporter cell lines, by InvivoGen (1 mentions) Hek blue detection, by InvivoGen (1 mentions) Lipopolysaccharides (lps), by Thermo Fisher Scientific (1 mentions) Poly 1 c, by Thermo Fisher Scientific (1 mentions) Ni chelating sepharose affinity chromatography, by GE Healthcare (1 mentions) Q ion exchange chromatography, by GE Healthcare (1 mentions) Endotoxin free ovalbumin ova, by InvivoGen (1 mentions) Aluminum hydroxide gel, by InvivoGen (1 mentions) Luciferase assay reagent, by Promega (1 mentions) Complete freund s adjuvant, by Thermo Fisher Scientific (1 mentions) Ovalbumin (ova), by InvivoGen (1 mentions)

Close spelling variants of this product

CpG-ODN (2216) (3 citations) CpG ODN 1826 adjuvant (1 citations)

6 protocols using cpg odn

Activation and Migration of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell migration assay was conducted using 24-well insert (Costar) with 5-µm pores. PBMCs from normal, healthy donors were activated with either CPG-ODN, a synthetic TLR9 agonist (Invitrogen), or Phytohemagglutinin (PHA) (Sigma) or anti-human IgM (Jackson Immuno Research) and anti CD40 monoclonal antibody, clone B-B20 (BioSite).
The activation status of the cells was then investigated by measuring CD69 expression on CD19+ and CD3+ PBMCs by Flow cytometry prior to migration assay. After 2 days of activation, 0.5 × 10⁶ PBMCs were added in the upper insert of the migration plates whereas 0.6 ml of supernatant from HIV-1 exposed or non-exposed FDCs were added in the lower insert. After 4 h at 37 °C, the inserts were removed, the cells collected from the lower wells and counted in a hemacytometer. Thereafter, the migrated cells were fixed with 2 % PFA and the frequency of CD19 and CD3 positive cells among migrated cells was evaluated.
The monoclonal antibodies (mAbs) used to identify and characterize the PBMCs in the migration assay are PE anti-CD69, FITC and PE anti-CD19, APC and FITC anti-CD3, PE and FITC isotype antibodies all purchased from BD Bioscience.

Sabri F., Prados A., Muñoz-Fernández R., Lantto R., Fernandez-Rubio P., Nasi A., Amu S., Albert J., Olivares E.G, & Chiodi F. (2016). Impaired B cells survival upon production of inflammatory cytokines by HIV-1 exposed follicular dendritic cells. Retrovirology, 13(1), 61.

+ Open protocol

+ Expand

Detailed Immunological Reagent Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

PMOC (>97% purity) was prepared as described in Supplementary Figure S1. TLR agonists including CpG ODN were purchased from Invitrogen (Carlsbad, CA, USA), rhTAK1-TAB1 or other protein kinases from SignalChem (Richimond, Canada), enzyme-linked immunosorbent assay (ELISA) kits and cytokines (TNF-α, IL-1α, HMGB-1) from R&D Systems (Minneapolis, MN, USA), and 2′,3′-O-(2,4,6-trinitrophenyl)-adenosine triphosphate (TNP-ATP) from Life Technology (Bangalore, India). Primary and secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA) or BioLegend (San Diego, CA, USA). Escherichia coli LPS and other materials were purchased from Sigma-Aldrich (St Louis, MO, USA) unless otherwise specified.

Park S.H., Kwak J.A., Jung S.H., Ahn B., Cho W.J., Yun C.Y., Na C.S., Hwang B.Y., Hong J.T., Han S.B, & Kim Y. (2017). Piperidylmethyloxychalcone improves immune-mediated acute liver failure via inhibiting TAK1 activity. Experimental & Molecular Medicine, 49(11), e392-.

+ Open protocol

+ Expand

TLR Reporter Cell Line Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

A TLR reporter cell line system that monitored TLR activity using secreted embryonic alkaline phosphatase (SEAP) induced by NF-κB and AP-1 activation was used. HEK-Blue™ Detection (InvivoGen) reacts with SEAP to turn the medium color purple/blue, and the color intensity can be measured with a spectrophotometer. The TLR reporter cell lines (InvivoGen) were cultured in DMEM containing 10% FBS and 1× antibiotics. When the cells reached 80% confluent, they were washed with PBS, and detection medium was added to harvest the cells using a scraper. Cells (5 × 10⁴ cells/well; 180 μl) were seeded in a 96-well plate, and 20 μl of PBS, samples, PAM3 (Invitrogen, Waltham, Massachusetts, USA), LPS (Invitrogen), poly I:C (Invitrogen), flagellin (Invitrogen), R848 (Invitrogen), or CpG-ODN (Invitrogen) was added to each well. Then, the cells were cultured at 37 °C, 5% CO₂, and 90% humidity for 20 h, and the absorbance at 630 nm was recorded.

Kim C.U., Jeong Y.J., Lee P., Lee M.S., Park J.H., Kim Y.S, & Kim D.J. (2022). Extracellular nucleoprotein exacerbates influenza virus pathogenesis by activating Toll-like receptor 4 and the NLRP3 inflammasome. Cellular and Molecular Immunology, 19(6), 715-725.

+ Open protocol

+ Expand

Synthesis and Purification of DP2-11 Peptide

Check if the same lab product or an alternative is used in the 5 most similar protocols

DP2-11 was synthesized by using fluorenyl-methyloxycarbonyl (Fmoc) chemistry at the Shanghai Science Peptide Biological Technology Co., Ltd. (Shanghai, China). The synthesized peptides were purified by high-performance liquid chromatography to obtain 95% purity, and their molecular weights were confirmed by mass spectrometry. The peptide powder was reconstituted in sterile water at a concentration of 10 mg/ml and stored at −20 °C. CpG ODN (murine TLR9 agonist, 5′-tccatgacgttcctgacgtt-3′) contains a full phosphorothioate backbone and was synthesized by Invitrogen Life Technologies. The CpG ODN powder was dissolved in sterile water at a concentration of 5 mg/ml and then stored at −20 °C until use. The aluminum hydroxide gel adjuvant (alum) was obtained from Brenntag Biosector, Frederikssund, Denmark. The NY-ESO-1 antigen, in which the endotoxin level was approximately 0.02 EU/μg, was purified via four process steps including Ni-chelating Sepharose affinity chromatography (GE Healthcare, Piscataway, NJ), excision of the Trx-His6-tag, removal of the Trx-His6-tag with second Ni-chelating Sepharose affinity chromatography, and Q-ion-exchange chromatography (GE Healthcare, Piscataway, NJ). Endotoxin-free ovalbumin (OVA) was purchased from InvivoGen.

Tian Y., Hu Q., Zhang R., Zhou B., Xie D., Wang Y., Zhang X, & Yang L. (2021). Rational design of innate defense regulator peptides as tumor vaccine adjuvants. NPJ Vaccines, 6, 75.

+ Open protocol

+ Expand

CpG-ODN Adjuvant Immunization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

CpG-ODN were purchased from Invitrogen or Genomics Biosci and Tech. Ovalbumin and aluminum hydroxide gel were purchased from Invivogen. Freund’s complete adjuvant and incomplete adjuvant were purchased from Thermo Scientific. Luciferase assay reagents were purchased from Promega.

Chuang T.H., Lai C.Y., Tseng P.H., Yuan C.J, & Hsu L.C. (2014). Development of CpG-Oligodeoxynucleotides for Effective Activation of Rabbit TLR9 Mediated Immune Responses. PLoS ONE, 9(9), e108808.

+ Open protocol

+ Expand

Hepatitis B Vaccine Formulations Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Twelve mice were randomly divided into 4 groups containing 3 mice each. Hepatitis B vaccine from Pasteur Institute of Iran (IRC 1228070570), which contained recombinant Hepatitis B Surface Antigen (rHBs-Ag) as immunogen and aluminum potassium sulfate (alum) as adjuvant was injected into three mice in group A. In group B, CFA (Sigma–Aldrich Co. Germany) was added to HBsAg plus alum. In group C, the mixture of HBsAg plus alum and oligodeoxynucleotides containing CpG motifs (CpG-ODN) (Invitrogen Co. USA) was used to immunize the mice, and the fourth group (D) were injected with Phosphate-Buffer-ed Saline (PBS) to serve as control. Therefore 4 groups were (HBsAg+Alum), (HBsAg+Alum)+FA, (HBsAg+ Alum)+CpG and control groups 22 (link),23 (link).

Khayyati Kohnehshahri M., Delirezh N, & Aghebati Maleki L. (2022). CpG-Containing Oligodeoxynucleotides and Freund Adjuvant in Combination with Alum Augment the Production of Monoclonal Antibodies Against Recombinant HBsAg. Avicenna Journal of Medical Biotechnology, 14(2), 125-131.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!