Supermex infrared ray passive sensor system

Seven to eight days after the infusion of RFRP-3, indirect calorimetry was performed using an O₂/CO₂ metabolism-measuring system for small animals (MK-5000RQ; Muromachi Kikai, Tokyo, Japan). The system monitored VO₂ (mL/min) and VCO₂ (mL/min) at 3-min intervals and calculated the RQ ratio (VCO₂/VO₂). The locomotor activity was simultaneously measured using the SUPERMEX infrared ray passive sensor system (Muromachi Kikai). The measurements were collected hourly or every 15 min over a 24 h period (light period: 09:00 a.m.–21:00 p.m., dark period: 21:00 p.m.–09:00 a.m.) after 24 h of habituation for 7 d after the infusion of RFRP-3. Energy expenditure was calculated using Equation (1) [59 ]: $energy expenditure \left(\raisebox{1ex}{$cal$}\!\left/ \!\raisebox{-1ex}{$\raisebox{1ex}{$kg$}\!\left/ \!\raisebox{-1ex}{$h$}\right.$}\right.\right)=V{O}_{2 }\left(\raisebox{1ex}{$mL$}\!\left/ \!\raisebox{-1ex}{$\raisebox{1ex}{$kg$}\!\left/ \!\raisebox{-1ex}{$h$}\right.$}\right.\right) \times \left\{3.815+\left(1.232 \times RQ\right)\right\}.$

Four weeks after injecting AAV-based vectors, indirect calorimetry was performed using an O₂/CO₂ metabolism-measuring system for small animals (MK-5000RQ; Muromachi Kikai, Tokyo, Japan). The system monitored VO₂ (ml/min) and VCO₂ (ml/min) at 3 min intervals and calculated the respiratory quotient (RQ) ratio (VCO₂/VO₂). Locomotor activity was simultaneously measured using the SuperMex infrared ray passive sensor system (Muromachi Kikai). Measurements were collected hourly over a 23 hr period (light period: 10:00–21:00, dark period: 21:00–9:00) after habituation for 30 min. Energy expenditure was calculated using the following equation: energy expenditure (cal/kg/hr) = VO₂ (ml/kg/hr) × [3.815 + (1.232 × RQ)] (Lusk, 1928 ).