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Opteia human il 10 elisa set 3555157

Manufactured by BD

The BD OptEIA Human IL-10 ELISA set #3555157 is a laboratory product designed for the quantitative measurement of human interleukin-10 (IL-10) in biological samples. The set includes the necessary components to perform an enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of IL-10 levels.

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2 protocols using opteia human il 10 elisa set 3555157

1

Cytokine Quantification by ELISA

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For determination of cytokine (IL-1β and IL-10) levels in the media, cells with or without 30 min pretreatment with 10 μM MCDNB and/or addition of 1 μg/mL LPS for 6 or 24 h were removed by centrifugation, and media was tested by ELISA according to the manufacturer's instructions (BD OptEIA Human IL-10 ELISA set #3555157 and BD OptEIA Human IL-1B ELISA set 2 #557953).
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2

Cytokine Quantification in Cell Media

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For determination of cytokine (IL-1β and IL-10) levels in the media, cells with or without 30 min pretreatment with 10 μM MCDNB and/or addition of 1 μg/mL LPS for 6 or 24 h were removed by centrifugation, and media was tested by ELISA according to the manufacturer’s instructions (BD OptEIA Human IL-10 ELISA set #3555157 and BD OptEIA Human IL-1B ELISA set 2 #557953).
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