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Cysteine is a non-essential amino acid that plays a crucial role in various biological processes. It is a sulfur-containing amino acid that can be synthesized in the human body. Cysteine is involved in the formation of proteins and enzymes, and it also contributes to the production of glutathione, an important antioxidant.

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6 protocols using cysteine

1

Biochemical Reagents Procurement Protocol

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SPI was obtained from Linyi Shansong Biological Products Co., Ltd. (Linyi, China). Cysteine and β-mercaptoethanol (β-ME) were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). Glycerol was obtained from Xilong Scientific Co., Ltd. (Shantou, China).
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2

Synthesis and Characterization of Zr-Based Hybrid Materials

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2-aminoterephthalic acid (ATA, 98%), tetracycline (TET, ≥98%), doxycycline (DOX, ≥98%), oxytetracycline (OTC, ≥98%), chlortetracycline (CTC, ≥98%), chloramphenicol (CAP, 98%), tyrosine (Tyr), histidine (His), serine (Ser), phenylalanine (Phe), arginine (Arg) and cysteine (Cys) were provided by Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Zirconium chloride (ZrCl4, 99%) was purchased from Beijing Yinuokai Technology Co., Ltd. (Beijing, China). Methacrylic acid (MAA, 99%), 2,2-azobisisobutyronitrile (AIBN, 99%) and ethylene glycol dimethacrylate (EGDMA, 98%) were obtained from TCI Development Co., Ltd. (Shanghai, China). N, N-dimethylformamide (DMF), ethanol, glucose, sucrose, MnCl2·4H2O, MgCl2·6H2O, Zn(NO3)2·6H2O, CaCl2, NaCl and KCl were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All reagents and chemicals used in this work were of at least analytical grade. The apparatuses utilized in this study are described in detail in the supporting information.
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3

Characterization of Carbon Fiber Materials

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All the chemicals were of analytical grade and used as purchased without further purification. CF (thickness of 1 mm) were supplied by Suzhou Taili New Energy Materials Ltd. Co (P.R. China). Sodium hydroxide, sodium chloride, glucose, dopamine, ascorbic acid, uric acid, cysteine, sucrose, lactose, and maltose were supplied by Macklin Inc, Shanghai, China.
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4

Highly Sensitive Enzymatic Biosensor

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All reagents were not purified further before use. Methylbenzene, hydrochloric acid (HCl), lysine (Lys), arginine (Arg), titanium tetrachloride (TiCl4), tetrabutyltitanate, gold (III) chloride (HAuCl4·3H2O), leucine (Leu), cysteine (Cys), proline (Pro), glycine (Gly), barium hydroxide octahydrate (Ba(OH)2·8H2O), formic acid, ethylene diamine tetraacetic disodium (EDTA−2Na), chloroplatinic acid (H2PtCl6), 3,3′,5,5′−tetramethylbenzidine (TMB, ≥99%), L−histidine (L−His), glutathione (GSH), glutamic acid (Glu), and fetal bovine serum were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Sodium borohydride (NaBH4), disodium hydrogen phosphate (Na2HPO4), p−Benzoquinone (p−BQ), isopropanol (IPA), potassium nitrate (KNO3), citric acid, ascorbic acid (AA), and sodium nitrate (NaNO3) were purchased from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China).
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5

Tannic Acid-Copper Complex Colorimetric Assay

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Tannic acid (TA), Cu(NO3)2·3H2O, ethanol, hydrogen peroxide (30 wt%), 3,3′,5,5′-tetramethylbenzidine (TMB), L-proline, glycine, cysteine, alanine, glutamic acid, NaCl, KCl, MnCl2·4H2O, CaCl2, glucose and L-glutathione reduced were purchased from Macklin Biochemical Co., Ltd. Ammonia solution (25–28 wt%) and formaldehyde (37–40 wt%) were purchased from Tianjin Zhiyuan Chemical Co., Ltd. Pluronic® F127 was purchased from Sigma-Aldrich. The qualitative filter paper was purchased from Whatman. All the reagents were used without further purification. Deionized water from a Milli-Q Plus system (Millipore) was used in all experiments.
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6

Synthesis and Characterization of Magnetic Nanoparticles

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FeCl3·6H2O and cysteine were purchased from Macklin (Shanghai, China). 1-Octadecene was purchased from TCI Co., Ltd (Shanghai, China). Ferumoxytol (Feraheme injection, AMAG Pharmaceuticals, MA) was used according to the instructions after purchase. Sodium oleate, ethyl alcohol, hexane, isopropanol and methylbenzene were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Chitosan was purchased from Aladdin (Shanghai, China). Phosphate buffer saline (PBS) and low glucose Dulbecco's modified Eagle's medium (DMEM) were purchased from HyClone (America). Penicillin/streptomycin and fetal bovine serum (FBS) were purchased from Gibco (America). Basic fibroblast growth factor (bFGF) was purchased from Invitrogen (America). The Cell Counting Kit-8 (CCK8) was purchased from Dojindo Co., Ltd (Japan).
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