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Anti il 6 antibody

Manufactured by BioLegend
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Sourced in United Kingdom, United States

The Anti-IL-6 antibody is a laboratory reagent used to detect and quantify the presence of the cytokine interleukin-6 (IL-6) in biological samples. It functions as a specific binding agent for IL-6, allowing researchers to measure IL-6 levels in their experiments.

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Lab products found in correlation

Mouse igg1, by BioLegend (2 mentions) Rat igg1, by BioLegend (2 mentions) Celltrace violet dye, by Thermo Fisher Scientific (1 mentions) Celltrace violet, by BioLegend (1 mentions) Facsaria system, by BD (1 mentions) Anti cd3 antibody, by BioLegend (1 mentions) Maz51, by Merck Group (1 mentions) Stattic, by MedChemExpress (1 mentions) Sw620, by Thermo Fisher Scientific (1 mentions) Dmem with high glucose content, by Thermo Fisher Scientific (1 mentions) Sw480, by Thermo Fisher Scientific (1 mentions) Sw480, by American Type Culture Collection (1 mentions) Sw620, by American Type Culture Collection (1 mentions) Ab47215, by Abcam (1 mentions) Human il 6 quantikine elisa kit d6050, by R&D Systems (1 mentions)

Close spelling variants of this product

Anti-IL-6 (18 citations) Anti-mouse IL-6 antibody (4 citations) Anti-human IL-6 antibody (3 citations) Rat anti-mouse IL-6 antibody (2 citations) APC anti-human IL-6 antibody (2 citations) Rat anti-mouse IL-6 monoclonal antibody (1 citations)

5 protocols using anti il 6 antibody

1

Murine Effector CD8+ T Cell Suppression by Tregs

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Spleens were harvested from FVB/N mice, minced and passed through 70 μm strainer. After washing cells were resuspended in PBS + 1% BSA and incubated with anti-CD3 (PerCP-Cy5.5; Cat# 100218), anti-CD4 (Brilliant Violet 711; Cat# 100447), anti-CD8 (Brilliant Violet 510; Cat# 100100752) and anti-CD25 (PE; Cat# 102008) antibodies. Cells were then sorted using FACS Aria system (BD Biosciences) for CD3+ CD8+ CD4- CD25- (effector CD8+ cells) and CD3+ CD8- CD4+ CD25hi (Treg cells). Effector CD8+ cells were labelled with CellTrace violet dye (Thermo Fischer) following manufacturer’s instructions. 96-well plates were coated in anti-CD3 antibody (1 μg/ml Biolegend, cat #100340), and splenocytes from CD-1 nude mice were added as antigen presenting cells (APCs). Isolated Tregs and CellTrace violet labelled CD8 cells were co-cultured in T cell media at 1:8 ratio for 5 days in the presence of either Kin1-WT or Kin1-NULL conditioned media (50% concentrated 2 X), and with or without anti-IL-6 antibody (20 μg/ml, Biolegend Cat#504512). Cells were then harvested from plates, added to 5 ml FACS tubes and analyzed by flow cytometry as detailed above.
Webb E.R., Dodd G.L., Noskova M., Bullock E., Muir M., Frame M.C., Serrels A, & Brunton V.G. (2023). Kindlin-1 regulates IL-6 secretion and modulates the immune environment in breast cancer models. eLife, 12, e85739.
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2

Blocking VEGF-C and IL-6 Signaling in Vessels

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MAZ51 (676492, EMD Millipore, Billerica, MA) was used to inhibit VEGF-C/VEGFR-3 signaling. Anti-IL-6R antibody (ab47215, Abcam, Cambridge, UK) and anti-IL-6 antibody (501109, BioLegend, San Diego) were used to inhibit IL-6/IL-6R signaling. Mouse IgG1 (400102, BioLegend, San Diego, CA) and rat IgG1 (400413, BioLegend, San Diego) antibodies were used as isotype controls for the IL-6/IL-6R inhibition experiments. To inhibit VEGFR-3, cultured vessels were primed for 1 hour with media containing MAZ51 (either 1 μM or 5 μM). Subsequently, MAZ51 was added to media containing VEGF-C (50 ng/mL) used to stimulate vessels over culture days 3 and 4. Vessels were washed with fresh media without VEGF-C or MAZ51 on day 5 and used for dextran diffusion analysis. To block vessels stimulated with IL-6 and in co-culture with CAFs, vessels were treated with anti-IL-6R antibody (either 5 μg/mL or 25 μg/mL) overnight from day 2 to day 3. Blocked vessels were then treated with media containing IL-6 (50 ng/mL) or left to co-culture with CAFs over days 3 and 4, and used on day 5 for dextran diffusion analysis. To neutralize IL-6 for the CAF co-cultures, vessels were treated with fresh medium containing 25 μg/mL of anti-IL-6 antibody from day 2 to day 4 and used on day 5 for dextran diffusion analysis.
Gong M.M., Lugo-Cintron K.M., White B.R., Kerr S.C., Harari P.M, & Beebe D.J. (2019). Human organotypic lymphatic vessel model elucidates microenvironment-dependent signaling and barrier function. Biomaterials, 214, 119225.
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3

Inhibiting STAT3 in Colorectal Cancer Cells

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We obtained human colorectal cancer cells (SW480, SW620, and SW48) from the American Type Culture Collection. The SW480, SW620, and SW48 cells were incubated in DMEM with high glucose content (Invitrogen, CA, USA) containing 10% FBS and 1% penicillin/streptomycin at 37 °C in 5% CO2 and 95% air. Stattic (MedChem Express, NJ, USA), a specific chemical inhibitor of STAT3; Anti-IL-6 antibody (Biolegend, CA, USA), a neutralizing antibody for IL-6.
Zhang X., Hu F., Li G., Li G., Yang X., Liu L., Zhang R., Zhang B, & Feng Y. (2018). Human colorectal cancer-derived mesenchymal stem cells promote colorectal cancer progression through IL-6/JAK2/STAT3 signaling. Cell Death & Disease, 9(2), 25.
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4

Inhibiting IL-6/IL-6R Signaling in HNC Cell Migration

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Anti-IL-6R antibody (ab47215, Abcam, Cambridge, UK) and anti-IL-6 antibody (501109, BioLegend, San Diego) were used to inhibit IL-6/IL-6R signaling. Mouse IgG1 (400102, BioLegend, San Diego, CA) and rat IgG1 (400413, BioLegend, San Diego) antibodies were used as isotype controls for the IL-6/IL-6R inhibition experiments. Vessels were treated with either media supplemented with anti-IL-6R antibody (5, 10, 20 μg/mL) or anti-IL-6 antibody (25, 50, 100 μg/mL) daily for the duration of migration experiments. Concentrations for both antibodies were based on previously optimized concentrations in the LumeNEXT platform [15] (link). The image analysis pipeline described above was used to assess migration differences in HNC cells with and without IL-6/IL-6R inhibition alongside their isotype antibody controls.
Yada R.C., Desa D.E., Gillette A.A., Bartels E., Harari P.M., Skala M.C., Beebe D.J, & Kerr S.C. (2023). Microphysiological head and neck cancer model identifies novel role of lymphatically secreted monocyte migration inhibitory factor in cancer cell migration and metabolism. Biomaterials, 298.
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5

Quantifying IL-6 Expression in EGFR-Mutant NSCLC

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To check the expression levels of IL-6 protein in 10 EGFR-mutant NSCLC cell lines [16, 17] , the supernatant IL-6 levels in the cell culture were measured by the enzyme-linked immunosorbent assay (ELISA), using Human IL-6 Quantikine ELISA Kit D6050 (R&D Systems, MN, USA). Sensitivities of each NSCLC cell to gefitinib and anti-IL-6 antibody (Biolegend, CA, USA) were evaluated by MTT assay.
Tamura T., Kato Y., Ohashi K., Ninomiya K., Makimoto G., Gotoda H., Kubo T., Ichihara E., Tanaka T., Ichimura K., Maeda Y., Hotta K, & Kiura K. (2018). Potential influence of interleukin-6 on the therapeutic effect of gefitinib in patients with advanced non-small cell lung cancer harbouring EGFR mutations. Biochemical and biophysical research communications, 495(1).
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