Anti tp53

Manufactured by Abcam

Sourced in United States, United Kingdom

Anti-TP53 is an antibody that binds to the TP53 protein. TP53 is a tumor suppressor protein that regulates cell division and plays a role in DNA repair. The antibody can be used in various research applications to study the TP53 protein and its functions.

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Lab products found in correlation

Protease inhibitor and phosphatase inhibitor, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Ecl kit, by Bio-Rad (1 mentions) Ripa buffer, by Merck Group (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Anti cdk1, by Abcam (1 mentions) Anti cdk2, by Abcam (1 mentions) Bx41 microscope, by Olympus (1 mentions) Goat anti mouse alexa 488 secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti human nuclear antigen, by Merck Group (1 mentions) Anti pax8, by Abcam (1 mentions) Ripa lysis buffer, by Thermo Fisher Scientific (1 mentions) Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Enhanced chemiluminescent reagent, by Merck Group (1 mentions) Anti gapdh, by Abcam (1 mentions) Anti egfr, by Abcam (1 mentions) Anti akt1, by Abcam (1 mentions) Anti myc, by Abcam (1 mentions)

3 protocols using anti tp53

Western Blotting for Cellular Proteins

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The RIPA buffer with protease inhibitor and phosphatase inhibitor (Sigma-Aldrich, USA) was used to extract total protein of 786-O cells. We used 10% SDS-PAGE to resolve the total protein and transferred the SDS-PAGE to PVDF membrane (Millipore, USA). Then, membranes were blocked by 5% non-fat milk and incubated with primary antibodies at 4°C for overnight. After washing, secondary antibody was used to incubated the membranes at room temperature for 2 h. Bands were visualized using an enhanced chemiluminescence (ECL) kit (Bio-rad, USA) and detected by BIO-RAD ChemiDoc MP Imaging System (Bio-Rad, USA).
Western blotting was performed using the following antibodies: anti-TOP2A, 1:1000 (Proteintech); anti-CDK1, 1:1000 (Abcam); anti-CDK2, 1:1000 (Abcam ); anti-CCNA1/2, 1:2000 (Abcam); anti-p-TP53, 1:1000 (Abcam); anti-TP53, 1:1000 (Abcam). Blotting membranes were stripped and probed again with anti-GAPDH antibodies (Santa) as a loading control.

Xiong Y., Yuan L., Chen L., Zhu Y., Zhang S., Liu X., Xiao Y, & Wang X. (2018). Identifying a Novel Biomarker TOP2A of Clear Cell Renal Cell Carcinoma (ccRCC) Associated with Smoking by Co-Expression Network Analysis. Journal of Cancer, 9(21), 3912-3922.

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Immunohistochemical Profiling of Tumor Samples

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Snap frozen tumor blocks were cryo-sectioned through the University of Michigan microscopy and imaging lab. Sections were fixed in 4% formaldehyde and permeabilized with 0.1% triton. Sections were stained with either mouse anti-human nuclear antigen (Millipore), anti-TP53 (abcam), anti-PAX8 (abcam), anti-human mitochondrial antibody (Life Technologies), anti-human CD34 (abcam) or anti-smooth muscle actin-Cy3 antibody (abcam) in 1:100 dilution. Of note, anti-human CD34 antibody used is predicted to detect mouse in addition to human CD34. Goat anti-mouse-Alexa 488 secondary antibody was then applied (Life Technologies). Images were obtained using an Olympus BX41 microscope. Paraffin imbedded tumor tissue was prepared, sectioned and stained through the University of Michigan Microscopy & Image Analysis core.

Coffman L.G., Burgos-Ojeda D., Wu R., Cho K., Bai S, & Buckanovich R.J. (2016). New models of hematogenous ovarian cancer metastasis demonstrate preferential spread to the ovary and a requirement for the ovary for abdominal dissemination. Translational research : the journal of laboratory and clinical medicine, 175, 92-102.e2.

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Western Blot Analysis of Protein Expression

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The predicted target protein expression was detected by western blotting. Briefly, total proteins were extracted from tendon tissue using RIPA lysis buffer (Applied Biosystems, MA, USA). Then, the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (Millipore, MA, USA). The membranes were blocked with 5% skimmed milk and incubated with primary antibodies anti-EGFR, anti-TP53, anti-AKT1, anti-MYC, and anti-GAPDH (1 : 1,000, Abcam, UK) at 4°C overnight. After washing with PBS, the membranes were incubated with a secondary antibody (1 : 1,000, Abcam). Enhanced chemiluminescent reagents (Millipore, MA, USA) were used to detect the protein bands. GAPDH was used as an internal control.

Qiu C., Chen Q., Hou Q, & Qi G. (2022). Systems Pharmacology and Molecular Docking Reveals the Mechanisms of Nux Vomica for the Prevention of Myasthenia Gravis. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 9043822.

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