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Pentaethylene glycol monooctyl ether c8e5

Manufactured by Anatrace

Pentaethylene glycol monooctyl ether (C8E5) is a non-ionic surfactant. It is a colorless, viscous liquid with a cloud point between 65-70°C. C8E5 is soluble in water and organic solvents.

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2 protocols using pentaethylene glycol monooctyl ether c8e5

1

Purification and Characterization of Type III Secretion Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemicals were from Sigma-Aldrich unless otherwise specified. Detergents n-dodecyl-maltoside (DDM), Lauryl Maltose Neopentyl Glycol (LMNG) and pentaethylene glycol monooctyl ether (C8E5) were from Anatrace. para-benzoylphenylalanine was from Bachem. SERVAGel™ TG PRiME™ 4-20% and 8-16 % precast gels were from Serva and 4–20% Mini-PROTEAN® TGX™ were from Bio-Rad. Primers are listed in Table S4 and were synthetized by Eurofins, Invitrogen or Sigma-Aldrich. Polyclonal rabbit anti-PrgK antibody was a kind gift of Thomas Marlovits, Vienna. Monoclonal M2 anti-FLAG antibody was from Sigma-Aldrich. Monoclonal anti-SipB and anti-InvJ antibodies were raised by Abmart. Secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo Fisher Scientific.
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2

Purification and Characterization of Type III Secretion Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemicals were from Sigma-Aldrich unless otherwise specified. Detergents n-dodecyl-maltoside (DDM), Lauryl Maltose Neopentyl Glycol (LMNG) and pentaethylene glycol monooctyl ether (C8E5) were from Anatrace. para-benzoylphenylalanine was from Bachem. SERVAGel™ TG PRiME™ 4-20% and 8-16 % precast gels were from Serva and 4–20% Mini-PROTEAN® TGX™ were from Bio-Rad. Primers are listed in Table S4 and were synthetized by Eurofins, Invitrogen or Sigma-Aldrich. Polyclonal rabbit anti-PrgK antibody was a kind gift of Thomas Marlovits, Vienna. Monoclonal M2 anti-FLAG antibody was from Sigma-Aldrich. Monoclonal anti-SipB and anti-InvJ antibodies were raised by Abmart. Secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo Fisher Scientific.
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