Ab181078

Antibodies for Western blotting were as follow: AP2S1 (ab128950, Abcam, 1:1000), APP‐full length and CTFs (A8717, Sigma, 1:1000), BACE1 (ab2077, Abcam, 1:1000), sAPP‐β (SIG‐39138, Biolegend, 1:500), VPS41 (ab181078, Abcam, 1:1000), VPS16 (17776‐1‐AP, Proteintech, 1:1000), GAPDH (Proteintech, 1:10000). Horseradish peroxidase‐conjugated anti‐mouse (AB_2722565, 1:5000) and anti‐rabbit (AB_2722564, 1:5000) secondary antibodies were purchased from Proteintech (Wuhan, China). Antibodies for immunofluorescence included: APP (MAB348, Sigma, 1:200), Aβ (MOAB‐2, Novus Biologicals, 1:1000), EEA1 (#2411, 1:200), GM130 (#12480, 1:200), RAB9 (#5118, 1:200), LAMP1 (#9091, 1:100) were purchased from Cell Signaling Technology. Alexa Fluor 488‐labeled Goat Anti‐Rabbit (A0428, 1:200), Cy3‐l‐abeled Goat Anti‐Rabbit (A0516, 1:200) and Alexa Fluor 647‐labeled Goat Anti‐Mouse (A0473, 1:200) secondary antibodies were purchased from Beyotime (Shanghai, China). Pitstop2 (HY‐115604), dynasore (HY‐13863), chloroquine (CQ, HY‐17589A), bafilomycin A1 (BafA1, HY‐100558), β‐secretase inhibitor LY2811376 (HY‐10472), α‐secretase inhibitor GI254023X (HY‐19956) and γ‐secretase inhibitor DAPT (HY‐13027) were purchased from MCE (Monmouth Junction, NJ, USA).

Antibodies used in this study include antiactin (MAB1501; EMD Millipore), anti–α-adaptin (sc-17771; Santa Cruz Biotechnology, Inc.), anti-CD63 (556019; BD), anti-DNA (61014; Progen), anti-Drp1 (611113; BD), anti-Eps15 (sc-534; Santa Cruz Biotechnology, Inc.), anti-Flag (anti-DDDDK, ab1257; Abcam), anti-Flag (F1804; Sigma-Aldrich), anti-GFP (ab6673; Abcam), anti-GFP (A6455; Invitrogen), anti-LAMP2 (sc-18822; Santa Cruz Biotechnology, Inc.), anti-LBPA (Z-SLBPA; Eschelon), anti-parkin (sc-32282; Santa Cruz Biotechnology, Inc.), anti-PDH E2/E3bp (ab110333; Abcam), anti-PINK1 (6946; Cell Signaling Technology), anti-PMP70 (SAB4200181; Sigma-Aldrich), anti-SDHA (ab14715; Abcam), anti-SNAP29 (ab138500; Abcam), anti-Stx17 (17815–1-AP; ProteinTech), anti-Stx17 (HPA001204; Sigma-Aldrich), anti-TIM23 (611222; BD), anti-TIP47 (Novus Biologicals), anti-TOM20 (sc-11414; Santa Cruz Biotechnology, Inc.), anti-UQCRFS1 (referred to herein as CIII-Rieske, ab14746; Abcam), anti-VAMP7 (sc-166394; Santa Cruz Biotechnology, Inc.), anti-VAMP8 (ab76021; Abcam), anti-VDAC1 (ab14734; Abcam), and anti-Vps41 (ab181078; Abcam). Unless otherwise specified, all reagents were purchased from Sigma-Aldrich. The TIP47 antibody was a gift from P. McPherson (McGill University, Montreal, Quebec, Canada).