Temed

10× ThermoPol^® Reaction Buffer, Deoxynucleotide (dNTP) Solution Mix, BSA, MgSO₄, Nt·BstNBI and Bst 2.0 WarmStart^® DNA Polymerase were purchased from NEB (Ipswich, MA). Enzyme concentrations were estimated from specific activities and molecular weights provded by the manufacturer. Ambion^® Buffer Kit (Tris-HCl and KCl), SYBR^™ Green II RNA Gel Stain, SYBR^™ Gold Nucleic Acid Gel Stain and Novex^™ TBE-Urea Sample Buffer (2×) were purchased from Thermo Fisher Scientific (Waltham, MA). Glycerol was purchased from Sigma (Burlington, MA). The UDAR templates, 10/60 DNA ladder, nuclease-free water and 1× TE buffer were purchased from IDT (Coralville, IA). The triggers were purchased from Eurofins Genomics (Louisville, KY). Oligonucleotide sequences can be found in the ESI (Table SI 1). 40% Acrylamide/Bis Solution, 29:1, Ammonium Persulfate (APS) and Urea were purchased from Bio-Rad (Hercules, CA). TEMED (Tetramethylethylenediamine) was purchased from Bio Basic Inc. (Amherst, NY). 10× TBE Buffer Concentrate (0.89 M Tris, 0.89 M boric acid, 20 mM EDTA, pH 8.3) was purchased from IBI Scientific (Dubuque, IA).