The candidate variant of MNS1 was validated by Sanger sequencing using specific primers flanking the variant of the candidate gene, MNS1-F: 5'-GAAACGTGATGCTGAAATAGCC-3' and MNS1-R: 5'-AGTTCCTCACAACAAACCTGTGATA-3'. The variant site in MNS1 was amplified through polymerase chain reaction (PCR) using Ex Taq DNA polymerase (Bio-Rad, Hercules, CA, USA) for all the family members. All the PCR products were sequenced using the ABI 3730 automated sequencer (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.
Ex taq dna polymerase
Manufactured by Bio-Rad
Sourced in United States
Ex Taq DNA polymerase is a thermostable DNA polymerase enzyme used in the polymerase chain reaction (PCR) process for the amplification of DNA sequences. It exhibits robust performance and high fidelity in DNA synthesis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ex taq dna polymerase
1
Validating Candidate Variant in MNS1
2
DNAH6 Gene Mutation Detection
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Forward and reverse PCR primers targeting mutation regions in the DNAH6 gene had the following sequences: c.6582 C > A: 5′-CCATCTTGTGCCCTGACAGT-3′ and 5′-GTCTGTCCACCCTCTGAAGC-3′; c.11258 G > A: 5′-GGAAGGAAATTCTGTTGTGTGCT-3′ and 5′-TGGGGATAGGGTGTGGCTATAA-3′; c.10025 G > A: 5′-CTGAATGCGAACAAGGGAGAC-3′ and 5′-AGATAGGGTTCCTTGCTGACG-3′; and c.2823dupT: 5′-TGAAAGGAATAAACAGGTGATGCT-3′ and 5′-ACTCTGAATGCCCCTCCTAAC-3′. These regions were amplified by PCR using Ex Taq DNA polymerase (Bio-Rad, Hercules, CA, USA) in all patients and their family members. PCR products were sequenced on a 3730XL system (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions.
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