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Nkx2.1 sc 13040

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NKX2.1 (sc-13040) is a mouse monoclonal antibody specific for the NKX2.1 protein. NKX2.1 is a transcription factor that plays a role in the development and function of the thyroid gland, lung, and forebrain.

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Lab products found in correlation

Igg conjugated alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Zombie uv fixable viability kit, by BioLegend (2 mentions) Lsr 2 flow cytometer, by BD (2 mentions)

Close spelling variants of this product

Sc-13040

2 protocols using nkx2.1 sc 13040

1

Quantifying GABAergic Neuron Purity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry analysis was performed at Day 35 of GABAergic interneuron differentiation in order to assess the purity of the cell population. Cells were dissociated and fixed with 4% paraformaldehyde and stained with the following antibodies: NKX2.1 (sc-13040, Santa Cruz Biotechnology), and GABA (A2052, Sigma). The secondary antibody was IgG-conjugated Alexa Fluor 488 (Life Technologies). Live and dead cells were identified using the Zombie UV Fixable Viability Kit (Biolegend). Unstained as well as IgG-conjugated Alexa Fluor 488 (Life Technologies) secondary antibody alone was used as a control and to gate positive populations. Cells were analyzed on a BD LSR II Flow Cytometer (BD Biosciences), and data were interpreted with FlowJo Version 10.
Wang C., Najm R., Xu Q., Jeong D.E., Walker D., Balestra M.E., Yoon S.Y., Yuan H., Li G., Miller Z.A., Miller B.L., Malloy M.J, & Huang Y. (2018). Gain of toxic Apolipoprotein E4 effects in Human iPSC-Derived Neurons Is Ameliorated by a Small-Molecule Structure Corrector. Nature medicine, 24(5), 647-657.
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2

Quantifying GABAergic Neuron Purity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry analysis was performed at Day 35 of GABAergic interneuron differentiation in order to assess the purity of the cell population. Cells were dissociated and fixed with 4% paraformaldehyde and stained with the following antibodies: NKX2.1 (sc-13040, Santa Cruz Biotechnology), and GABA (A2052, Sigma). The secondary antibody was IgG-conjugated Alexa Fluor 488 (Life Technologies). Live and dead cells were identified using the Zombie UV Fixable Viability Kit (Biolegend). Unstained as well as IgG-conjugated Alexa Fluor 488 (Life Technologies) secondary antibody alone was used as a control and to gate positive populations. Cells were analyzed on a BD LSR II Flow Cytometer (BD Biosciences), and data were interpreted with FlowJo Version 10.
Wang C., Najm R., Xu Q., Jeong D.E., Walker D., Balestra M.E., Yoon S.Y., Yuan H., Li G., Miller Z.A., Miller B.L., Malloy M.J, & Huang Y. (2018). Gain of toxic Apolipoprotein E4 effects in Human iPSC-Derived Neurons Is Ameliorated by a Small-Molecule Structure Corrector. Nature medicine, 24(5), 647-657.
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