Acetic acid (AA), acetonitrile (ACN), atovaquone, dimethyl sulfoxide (DMSO), formic acid (FA), methanol (MeOH), and water were purchased from Fisher Scientific (Pittsburgh, PA). Glycine, 2,2-d2-Glycine, leucine, 1-13C, 15N-leucine, 1,2-13C2-leucine, 2-13C, 15N-leucine, formaldehyde, formaldehyde-d2 solution, sodium cyanoborohydride, sodium cyanoborodeuteride, triethylammonium bicarbonate buffer (TEAB, 1.0 M), tris(2-carboxy-ethyl) phosphine hydrochloride (TCEP), RPMI-1640 medium were purchased from Sigma-Aldrich (St. Louis, MO). Maltooctaose was purchased from Toronto Research Chemicals (Toronto, Canada). PNGase F was purchased from Promega (Madison, WI). Bovine thyroglobulin (BTG) and human IgG subtypes were purchased from Thermo Fisher Scientific (Rockford, IL). ECC1 cells were obtained from ATCC (Manassas, VA). Oasis HLB 1cc cartridges were purchased from Waters Corporation (Milford, MA). Microcon-30kDa centrifugal filters (30K MWCO) were purchased from Merck Millipore Ltd. (Darmstadt, Germany). PolyGLYCOPLEX A™ beads (3 μm) were purchased from PolyLC Inc. (Columbia, MD). Fused silica capillary tubing (i.d., 75 μm, o.d., 375 μm) was purchased from Polymicro Technologies (Phoenix, AZ). All reagents were used without additional purification.
Ecc1 cells
Manufactured by American Type Culture Collection
The ECC1 cells are an endometrial carcinoma cell line derived from human endometrial adenocarcinoma tissue. These cells can be used for various research applications, including the study of endometrial cancer and related cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Sodium cyanoborodeuteride, by Merck Group (1 mentions)
Leucine, by Merck Group (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Fused silica capillary tubing, by Molex (1 mentions)
Microcon 30 kda centrifugal filters 30k mwco, by Merck Group (1 mentions)
15n leucine, by Merck Group (1 mentions)
Oasis hlb 1 cc cartridges, by Waters Corporation (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
Sodium cyanoborohydride, by Merck Group (1 mentions)
Pngase f, by Promega (1 mentions)
Phenol red free dmem f12, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
2 protocols using ecc1 cells
1
Glycopeptide Analysis by Mass Spectrometry
2
Cell Line Characterization and Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HEC1A, AN3CA, and KLE cell lines were provided by Dr. Churl Ki Min (Ajou University, Suwon, South Korea), and the RL95-2 cell line was provided by Dr. Jong-Min Kim (Dong-A University, Pusan, Korea). The Ishikawa cell line was a gift from Dr. Hyung Sik Kim (Pusan National University, Pusan, Korea). The EM-E6/E7/hTERT cell line was originated by Mizumoto and colleagues [35 (link)] and obtained from Dr. Paul J. Goodfellow (Ohio State University, Columbus, OH). ECC-1 cells were purchased from the ATCC. All cell lines were maintained in Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM /F12; Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 1% penicillin-streptomycin (Gibco) at 37°C in a humidified atmosphere of 5% CO2. For E2 treatment, cells were maintained in phenol red-free DMEM/F12 (Gibco) with 5% charcoal-stripped FBS for 1 day followed by serum-free medium for 24 h before the experiment. The E2 and E2-BSA (bovine serum albumin) were purchased from Sigma. E2-BSA was prepared according to the protocol for removing free E2 as previously described [36 (link)]. Cycloheximide (CHX) and N-acetyl-leucinyl-leucinyl-norleucinal (ALLN) were purchased from Sigma.
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