HPLC analysis of DOX was performed with a Waters e 2695 Separations Module HPLC system (Waters Corporation, USA) connected to an autosampler, and a Waters 2998 Photodiode Array (PDA) Detector as per British pharmacopeia (Pharmacopoeia, 1999 ). Stationary phase comprised of Hypersil™ C18 ODS (150 × 4.6 mm; 5 μm, Thermo Fischer Scientific Inc., USA) column, operated at ambient temperature. Mobile phase consisted of solution A and solution B (1:1 ratio), where solution A comprised of 5 parts of methanol and 45 parts of acetonitrile, and solution B comprised of 2.88 g L−1 of sodium lauryl sulfate and 2.30 g L−1 of phosphoric acid in water. Mobile phase was pumped at a flow rate of 1 mL min−1 with a detection wavelength of 480 nm. Ten microliters of sample solution was injected in each run with a total chromatographic run time of 20.0 min. All samples were passed through a 0.22-μm syringe filter prior to HPLC analysis. Data acquisition, data handling, and instrument control were performed by Empower® software v 2.0.
E 2695 separations module hplc system
Manufactured by Waters Corporation
Sourced in United States
The E 2695 Separations Module is a high-performance liquid chromatography (HPLC) system developed by Waters Corporation. The core function of this equipment is to perform automated liquid chromatographic separations, allowing for the analysis and purification of a wide range of chemical compounds.
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Lab products found in correlation
2 protocols using e 2695 separations module hplc system
1
HPLC Analysis of Doxorubicin
2
HPLC Analysis of Cinnamic Acid in Cassia
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HPLC was performed using a Waters E2695 Separations Module HPLC system (Milford, MA, USA) for detection and extraction of cinnamic acid from C. cassia extracts. A PDA detector (Waters) was used for detection of the components and the separation was carried out with a Phenomenex Gemini C18 column (250 × 4.6 mm, 5 μm) (Waters). The C. cassia extract was dissolved with 50% methanol, and 10 μL of it was injected into the column. Cinnamic acid standard for HPLC analysis was obtained from Sigma–Aldrich (St. Louis, MO, USA). To detect cinnamic acid, the mobile phase was composed of distilled water with 0.1% (v/v) trifluoro acetic acid (TFA) (solvent A) and acetonitrile (ACN) with 0.1% (v/v) TFA (solvent B) (Figure 1 ). The gradient program was 0–30 min, 10–90% B; 30–35 min, 90% B; 35–40 min, 90–10% B; 40–50 min, 10% B at a flow rate of 0.7 mL/min at 280 nm.
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