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Poly 1 c lyovec

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Poly(I:C)/LyoVec is a lyophilized complex of the synthetic double-stranded RNA analog polyinosinic-polycytidylic acid (Poly(I:C)) and the transfection reagent LyoVec. Poly(I:C) is a Toll-like receptor 3 (TLR3) agonist used to stimulate innate immune responses.

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Lab products found in correlation

Poly 1 c, by InvivoGen (2 mentions) Poly da dt lyovec, by InvivoGen (2 mentions) Cd86 fitc, by Thermo Fisher Scientific (1 mentions) Cd16 apc, by Thermo Fisher Scientific (1 mentions) Cd16 fitc, by Thermo Fisher Scientific (1 mentions) Cd40 fitc, by Thermo Fisher Scientific (1 mentions) Cd163 pe, by BD (1 mentions) Cd68 pe, by BD (1 mentions) Dc sign fitc, by BD (1 mentions) Cd206 apc, by BD (1 mentions) Lyovec, by InvivoGen (1 mentions) Ssrna40, by InvivoGen (1 mentions) Scrambled sirna, by Thermo Fisher Scientific (1 mentions) Gardiquimod, by InvivoGen (1 mentions) Cd206 pe, by BioLegend (1 mentions) Cd40 pe cy7, by BD (1 mentions) Cd14 fitc, by Thermo Fisher Scientific (1 mentions) Cd11c apc, by BD (1 mentions) Cd14 apc, by BD (1 mentions) Cd14 pe, by BD (1 mentions)

Close spelling variants of this product

Poly(I:C) (721 citations) LyoVec (73 citations) Poly(I:C) (HMW)/LyoVec (10 citations) Poly(I:C) (LMW)/LyoVec (7 citations) POLY I (2 citations)

5 protocols using poly 1 c lyovec

1

TLR3/RIG-I Agonist Treatment of C8-D1A Cells

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Twenty-four-hour cultures of C8-D1A cells were treated with a TLR3 agonist, viral dsRNA substitute—poly(I:C) (InvivoGen, San Diego, CA, USA)—or RIG-I/MDA-5 agonist as a control—poly(I:C)/LyoVec (InvivoGen). At the time of treatment, the culture medium was replaced with fresh medium containing poly(I:C) or poly(I:C)/LyoVec. The 10 μg/ml poly(I:C) and 1 μg/ml poly(I:C)/LyoVec concentrations were determined empirically for further experiments.
Mielcarska M.B., Bossowska-Nowicka M., Gregorczyk-Zboroch K.P., Wyżewski Z., Szulc-Dąbrowska L., Gieryńska M, & Toka F.N. (2019). Syk and Hrs Regulate TLR3-Mediated Antiviral Response in Murine Astrocytes. Oxidative Medicine and Cellular Longevity, 2019, 6927380.
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2

Investigating Immune Cell Responses to TLR Agonists

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Poly I:C, Gardiquimod, ssRNA40, PolyI:C/Lyovec and Lyovec (vehicle control) were purchased from Invivogen (San Diego, CA). Human antibodies; CD16 APC, CD16 FITC, CD14 FITC, CD40 FITC and CD86 FITC were purchased from eBioscience (San Diego, CA). Antibodies CD14 APC, CD14 PE, CD40 PE-Cy7, CD163 PE, CD11c APC, CD68 PE, CD206 APC, DC-SIGN-FITC and isotype control antibodies were purchased from BD Pharmingen (Franklin Lakes, NJ). Human antibodies CD40 Alexa 700, CD68 PE-Cy7, Brilliant violet LAP (TGFβ), CD206-PE, CD16 Alexa-700 were purchased from Biolegend (San Diego, CA). Transwell-6 system with a 0.4-μm porous membrane was purchased from BD Biosciences, Franklin Lakes, NJ. TLR3, TLR7, TLR8 siRNA and scrambled siRNA were purchased from Ambion Life Technologies (Carlsbad, CA).
Saha B., Kodys K., Adejumo A, & Szabo G. (2017). Circulating and exosome packaged single-stranded Hepatitis C RNA induce monocyte differentiation via TLR7/8 to polarized macrophages and fibrocytes (128/130 characters). Journal of immunology (Baltimore, Md. : 1950), 198(5), 1974-1984.
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3

Monitoring Immune Response Signaling Pathways

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RNA polymerase subunit C32 and SRSF1 antibodies were purchased from Santa Cruz Biotechnology: Pol III RPC32 antibody (H-9): sc-48365, SF2/ASF Antibody (P-15): sc-10254. Poly (dA:dT)/LyoVec, poly (I:C)/LyoVec, and 5′ppp-dsRNA were purchased from Invivogen. Human IFN-β ELISA kits were obtained from PBL Interferon Source. Human IL-6, TNFα, and IL-1β ELISA Duoset were purchased from R&D Systems. ML-60218 was purchased from SYMANSIS.
Xue F., Li X., Zhao X., Wang L., Liu M., Shi R, & Zheng J. (2015). SRSF1 Facilitates Cytosolic DNA-Induced Production of Type I Interferons Recognized by RIG-I. PLoS ONE, 10(2), e0115354.
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4

Cell Culture and Infection Protocols

Cited 1 time
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HeLa cells, WT (wild-type) and TREX1 KO mouse embryonic fibroblasts (MEFs), and HEK293T were cultured in supplemented DMEM and mouse J774 cells cultured in complete media for macrophages, as described earlier (25 (link)). Mouse BM1.11 cells (26 (link)) and human OE-E6/E7 (oviduct epithelial cells) (27 (link)) were cultured in supplemented F12-DMEM as described (25 (link)). Poly dA:dT/LyoVec (100 µg/ml), Poly I:C/LyoVec (50 µg/ml) and 2’3’ cGAMP (1 mg/ml) were purchased from Invivogen. Carbenoxolone (working concentration 0.2 mM) was purchased from SIGMA. pBluscript vector (1–2.5 µg) was used as immunostimulatory DNA (ISD). cDNA constructs for human cGAS (pCMV-cGAS) and STING (pCMV-STING) were purchased from Origene. C. muridarum, C. trachomatis D and L2 were propagated in McCoy cells and infections performed at 1 MOI or as indicated, as previously described (25 (link)).
Zhang Y., Yeruva L., Marinov A., Prantner D., Wyrick P., Lupashin V, & Nagarajan U.M. (2014). The DNA sensor, cyclic GMP-AMP synthase (cGAS) is essential for induction of IFN beta during Chlamydia trachomatis infection. Journal of immunology (Baltimore, Md. : 1950), 193(5), 2394-2404.
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5

Mimicking Rhinovirus Infection with Poly(I:C)

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In this study, we used polyinosinic:polycytidylic acid (Poly(I:C); dsRNA)) as a viral mimic. During replication, RV produces intermediate, dsRNA molecules that bind to TLR‐3 within the endosomes or MDA5/RIG‐I in the cytoplasm. RV‐induced production of interferons and pro‐inflammatory cytokines are mainly dependent on the dsRNA rather than the viral proteins, indicating that UV‐inactivated RV does not induce any response in epithelial cells.10 By using TLR‐3 agonist Poly(I:C) high molecular weight (InvivoGen) (10 μg/mL) or the MDA5/RIG‐I agonist Poly(I:C)‐lyovec (InvivoGen) (0.25 μg/ml), we mimic RV infection with a lower variability in the cytokine response compared with a RV infection to obtain more standardized experiments.
Cerps S., Sverrild A., Ramu S., Nieto‐Fontarigo J.J., Akbarshahi H., Menzel M., Andersson C., Tillgren S., Hvidtfeldt M., Porsbjerg C, & Uller L. (2022). House dust mite sensitization and exposure affects bronchial epithelial anti‐microbial response to viral stimuli in patients with asthma. Allergy, 77(8), 2498-2508.
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