On the seventh day after myocardial infarction, B-mode and M-mode echocardiography was performed with a Vevo 770 machine (Visual Sonics Toronto, Canada). The end-diastolic left ventricular diameter (LVIDd), end-systolic left ventricular diameter (LVIDs), end-diastolic left ventricular volume (LV Vold), end-systolic left ventricular volume (LV Vols), left ventricular fractional shortening (LVFS), left ventricular ejection fraction (LVEF) were measured.
Vevo 770 machine
Manufactured by Fujifilm
Sourced in Canada
The Vevo 770 is a high-performance, high-resolution, real-time, in vivo micro-imaging system. The Vevo 770 provides non-invasive, high-resolution visualization and quantification of anatomical, functional, and molecular information from small animal models.
Automatically generated - may contain errors
4 protocols using vevo 770 machine
1
Echocardiographic Assessment Post-Myocardial Infarction
2
Cardiac Function Assessment After Surgery
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Cardiac function was assessed at 6 days after the surgery by Vevo770 machine using RMV 707B probe (VisualSonics, Fujifilm). Left ventricular internal diastolic dimension (LVIDd) and left ventricular internal systolic dimension (LVIDs) were measured from M‐mode images obtained by left ventricular short‐axis view. The averages of three to five measured values per mouse by echocardiography were used for statistical analysis. Mouse blood pressure was measured at 5 days after the surgery by tail cuff method using BP‐2000 Blood Pressure Analysis System™ (Visitech Systems, Inc.). The median of systolic blood pressure was calculated from 15 to 20 measured values per each mouse.
3
Cardiac Function Assessment in Mice
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Echocardiography was performed with a Visual Sonics Vevo 770 machine using a 30 MHz high‐frequency transducer (Visual Sonics). All mice were anaesthetized using 2.0% isoflurane. The left ventricular ejection fraction (LVEF), fractional shortening (FS), peak E, peak A, early (E′), late (A′), left ventricular end‐diastolic diameter (LVEDD), left ventricular posterior wall diameter (LVPWD) and electrocardiogram were measured. The ratio of early‐to‐late mitral flow velocity (E/A) and diastolic velocity ratio (E′/A′) was calculated.
4
Echocardiographic Assessment of Cardiac Function
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Transthoracic echocardiography was performed using Visual Sonics Vevo 770 machine using a 30 MHz high frequency MS400 transducer (Visual Sonics, Canada). Anesthesia (5% isoflurane) was administered, and the mice remained under general anesthesia with continuous administration of 2% isoflurane during echocardiogram acquisition. Indices of systolic function were obtained from long-axis M-mode scans. The collected parameters included left ventricular ejection fraction (LVEF), fractional shortening (FS), left ventricular end-diastolic diameter (LVEDd), left ventricular end-diastolic volume (LVEDV), ratio of early to late diastolic mitral flow velocities (E/A), ratio of early to late diastolic mitral annular velocities (E’/A’), and ratio of early diastolic transmittal flow velocity to early diastolic mitral annular velocity (E/E’) were calculated.
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