Thy1 stop yfp

Wild type C57BL/6J mice and the following mutant mouse lines were obtained from The Jackson Laboratory: Pten^flox (stock # 004597) (Groszer et al., 2001 (link)), p53^flox (stock # 008462) (Marino et al., 2000 (link)), p21 KO (stock # 003263) (Brugarolas et al., 1995 (link)), mGfap-Cre line 77.6 (stock # 024098) (Gregorian et al., 2009 (link)), Thy1-STOP-YFP (stock # 005630) (Buffelli et al., 2003 (link)), and Rosa-tdT (stock # 007914) (Madisen et al., 2010 (link)). Unless otherwise stated, both male and female mice at 8 weeks and older were used for all experiments. All mice were housed under a controlled temperature and a 12-h light/dark cycle with free access to water and food in an animal facility at UT Southwestern. The sample sizes were empirically determined. The experiments were not randomized and the researchers were not blinded to the allocation of animals during experiments and outcome assessment. All experimental procedures and protocols were approved by the Institutional Animal Care and Use Committee at UT Southwestern.