Take3 trio microplate

Peripheral venous whole blood was drawn from TBI subjects no later than 24h of injury onset. Blood was collected into PAXgene^® Blood RNA tubes (Becton-Dickinson). Immediately after blood collection, tubes were inverted 8–10 times and stored at −80°C until analysis which was less than one year from collection. Whole blood was collected in serum separator tubes, centrifuged at 4000 g, aliquoted into microcentrifuge tubes within 1h of collection, and stored at −80°C until analysis.
PAXgene^® Blood RNA tubes were thawed at room temperature overnight (16–20h) prior to RNA extraction. The PAXgene Blood RNA kit (Pre-Analytix) was used to purify/extract intracellular RNA, per manufacturer’s instructions. RNA concentration and quality was determined by absorbance using a Take3 Trio Microplate (BioTek^®) read on a Syntek Hybrid Plate Reader and analyzed using Gen5 (BioTek) software. A260/A280 values between 1.8 and 2.2 were considered acceptable RNA quality.

PAXgene® Blood RNA tubes were thawed overnight (16–20 hrs) at room temperature prior to RNA extraction. The PAXgene Blood RNA kit (Pre-Analytix) was used to purify/extract intracellular RNA, per manufacturer’s instructions. RNA concentration and quality was determined by absorbance using a Take3 Trio Microplate (BioTek®) read on a Syntek Hybrid Plate Reader, and analyzed using Gen5 (BioTek) software. A260/A280 values between 1.8 and 2.2 were considered acceptable RNA quality.