Animals were sacrificed in the diestrus phase of the estrous cycle (13–15 weeks of age). Animals were deeply anesthetized using sevoflurane (Abbot Laboratories, North Chicago IL) and transcardially perfused with 100 ml of 100 mM PBS wash followed by 100–150 ml of 4 % paraformaldehyde buffered in PBS (Sigma-Aldrich, St. Louis, MO). Brains were dissected and post-fixed in 4 % paraformaldehyde for 30– 60 minutes. After post-fixation, 200 μm thick coronal slices were cut using a rat brain matrix (ASI Instruments, Warren, MI). Serial coronal slices were then washed in PBS 3×, notched for orientation, and placed in tissue cell culture plates (24 well plate; Corning, Tewksbury MA) until further processing.
Tissue cell culture plates 24 well plate
Manufactured by Corning
Corning's 24 well tissue cell culture plates are laboratory equipment designed for culturing and growing cells in a controlled environment. The plates feature 24 individual wells, each with a uniform surface area, to facilitate parallel cell culture experiments. The plates are made of high-quality, tissue culture-treated polystyrene, ensuring optimal cell attachment and growth.
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Lab products found in correlation
2 protocols using tissue cell culture plates 24 well plate
1
Perfusion and Tissue Preparation
2
Perfusion and Brain Slice Preparation
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Animals were sacrificed between 14 and 17 months of age following repeated OSA of MPH. Animals were deeply anesthetized using sevoflurane (Abbot Laboratories, North Chicago, IL) and transcardially perfused using methodology adapted from Roscoe et al.46 (link). Brains were dissected and post-fixed in 4% paraformaldehyde for 10 minutes. A rat brain matrix (ASI Instruments, Warren, MI) was used to cut 1 mm thick coronal slices, which were washed 3x in PBS, notched for orientation, and placed in tissue cell culture plates (24 well plate; Corning, Tewksbury, MA).
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