Dynamic light scattering (DLS) measurements were performed using a Malvern Nano ZetaSizer (Malvern Instruments, Malvern UK). Data was processed using Dispersion technology software (v 7.13, Malvern instruments, Malvern UK) using the multiple narrow modes algorithm based upon a non-negative least square fit to calculate the hydrodynamic diameter and the PolyDispersity Index (PDI). The z-average is an intensity-based overall average hydrodynamic size based on a specific fit to the raw correlation function data. The PDI is a measure of the degree of polydispersity of the sample. Zeta potential measurements were performed which measure the surface charge of particles by measuring their velocity while they are moving due to electrophoresis. Samples were diluted to c.5 mM and analysis was performed at 25°C. All measurements were performed three times, the average with standard deviation was reported.
Dispersion technology software
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Dispersion Technology Software is a powerful tool developed by Malvern Panalytical to analyze and characterize the dispersion and particle size distribution of various materials. It provides comprehensive data analysis and reporting capabilities to aid in understanding the behavior and properties of dispersed systems.
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Lab products found in correlation
Zetasizer nano z, by Malvern Panalytical (4 mentions)
Zetasizer nano zs90, by Malvern Panalytical (3 mentions)
Malvern zetasizer nano, by Malvern Panalytical (2 mentions)
Zetasizer nano s, by Malvern Panalytical (2 mentions)
Zetasizer nano, by Malvern Panalytical (2 mentions)
Dts0012, by Malvern Panalytical (2 mentions)
Zetasizer nano series, by Malvern Panalytical (2 mentions)
Nano z, by Malvern Panalytical (2 mentions)
Malvern nano zs, by Malvern Panalytical (1 mentions)
532 nm laser, by Malvern Panalytical (1 mentions)
Zetasizer nano zs machine, by Malvern Panalytical (1 mentions)
Henelaser source, by Malvern Panalytical (1 mentions)
Uv 1800 uv vis spectrophotometer, by Horiba (1 mentions)
Digital micrograph, by Ametek (1 mentions)
Zetasizer ultra instrument, by Malvern Panalytical (1 mentions)
300 mesh au grid, by Ted Pella (1 mentions)
Milli q water, by Merck Group (1 mentions)
Zetasizer nano zs90 particle sizer, by Malvern Panalytical (1 mentions)
28 protocols using dispersion technology software
1
Characterization of Nanoparticle Size and Surface Charge
2
Characterization of Lipid Nanocarriers
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The hydrodynamic diameter, PDI, and zeta potential of the CFE-NLCs and blank NLCs were determined using a Zetasizer Nano ZS instrument coupled with Dispersion Technology software (version 5) (Malvern Instruments Ltd, Malvern, Worcestershire, UK).23 (link)
Before measurement, the samples were diluted with ultrapure water to a 1:300 dilution. All measurements were repeated at least three times at 25°C.
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The hydrodynamic diameter, PDI, and zeta potential of the CFE-NLCs and blank NLCs were determined using a Zetasizer Nano ZS instrument coupled with Dispersion Technology software (version 5) (Malvern Instruments Ltd, Malvern, Worcestershire, UK).23 (link)
Before measurement, the samples were diluted with ultrapure water to a 1:300 dilution. All measurements were repeated at least three times at 25°C.
3
Characterization of Iron Nanoparticles via DLS
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Dynamic
light scattering (DLS) measurements were performed at 25 °C using
a Malvern NanoZS (Malvern Instruments, Malvern U.K.). Typically, the
iron concentration of each sample was between 0.25 and 2 mM. Analysis
is done using Dispersion Technology software (v. 4.10, Malvern Instruments;
Worcestershire, U.K.) using the multiple narrow modes algorithm based
upon a non-negative least-squares fit. For all suspensions presented,
the PDI values were low and the cumulants fit matched
the data very well; hence, the Z-ave value is taken
as a measure of dhyd throughout. Zeta
potential (ζ) measurements were performed at 25 °C on a
Malvern NanoZS (Malvern Instruments, Malvern U.K.) using the M3-PALs
approach. All DLS and zeta potential measurements were run in triplicate.
In all cases, the three values were in very close agreement, and the
average was used.
light scattering (DLS) measurements were performed at 25 °C using
a Malvern NanoZS (Malvern Instruments, Malvern U.K.). Typically, the
iron concentration of each sample was between 0.25 and 2 mM. Analysis
is done using Dispersion Technology software (v. 4.10, Malvern Instruments;
Worcestershire, U.K.) using the multiple narrow modes algorithm based
upon a non-negative least-squares fit. For all suspensions presented,
the PDI values were low and the cumulants fit matched
the data very well; hence, the Z-ave value is taken
as a measure of dhyd throughout. Zeta
potential (ζ) measurements were performed at 25 °C on a
Malvern NanoZS (Malvern Instruments, Malvern U.K.) using the M3-PALs
approach. All DLS and zeta potential measurements were run in triplicate.
In all cases, the three values were in very close agreement, and the
average was used.
4
Exosome Particle Size Analysis via DLS
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A dynamic light scattering (DLS) system equipped with a 532-nm laser (Malvern Instruments, Malvern, UK) was used for exosome particle size analysis. Samples were diluted before analysis (10 μL, 1:100 diluted in PBS) and each sample was measured three times. Data was collected and analyzed by Dispersion Technology Software (Malvern Instruments). The size distribution was measured by signal intensity and the Z-average diameter obtained from the autocorrelation function using the general-purpose mode.
5
Cubosomal Size Characterization
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The size distribution of cubosomes was measured using a Zetasizer Nano ZS90 (Malvern Instruments, Malvern, UK). The samples were diluted 50-fold with water to 2% before measurement. Cubosomal size was analyzed by the Dispersion Technology Software provided by Malvern Instruments.
6
Dynamic Light Scattering Particle Size Analysis
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The scattered light intensity was measured using a Malvern Zetasizer Nano ZS (Malvern, Herrenberg, Germany) equipped with a 633-nm He-Ne laser and operating at an angle of 173°. The Dispersion Technology Software, from Malvern, was used to collect and analyze the data. The measurements were made at a controlled temperature of 25 °C, on 500 μl of each sample measured in single-use polystyrene cuvettes (Fisher Emergo, Landsmeer, The Netherlands) with a pathlength of 10 mm. The intensity size distribution, the Z-average diameter (Z-ave) and the polydispersity index (PdI) were obtained from the autocorrelation function. The default upper threshold of 0.01, lower threshold of 0.05, and the default filter factor of 50% were used.
7
Particle Formation Monitoring via DLS
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The formation of particles
in the stability samples was monitored using 90° DLS (Zetasizer
Nano ZS90, Malvern Instruments, Ltd., Westborough, MA). Approximately
1 mL of the reconstituted Mb sample was placed in a polystryrene cuvette
(VWR) and analyzed with a path length of 10 mm at 25 °C. Triplicate
samples for each formulation were recorded 3 times using an automatic
mode for the selection of best number of subruns. The z-average diameter (nm) was calculated from the correlation function
using the Dispersion Technology Software supplied with the instrument
(Version 4.20, Malvern, Westborough MA).
in the stability samples was monitored using 90° DLS (Zetasizer
Nano ZS90, Malvern Instruments, Ltd., Westborough, MA). Approximately
1 mL of the reconstituted Mb sample was placed in a polystryrene cuvette
(VWR) and analyzed with a path length of 10 mm at 25 °C. Triplicate
samples for each formulation were recorded 3 times using an automatic
mode for the selection of best number of subruns. The z-average diameter (nm) was calculated from the correlation function
using the Dispersion Technology Software supplied with the instrument
(Version 4.20, Malvern, Westborough MA).
8
Nanoparticle Size Characterization by DLS
9
Characterization of Nanoparticle Size and Surface Charge
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Dynamic light scattering (DLS) measurements were performed using a Malvern Nano ZetaSizer (Malvern Instruments, Malvern UK). Data was processed using Dispersion technology software (v 7.13, Malvern instruments, Malvern UK) using the multiple narrow modes algorithm based upon a non-negative least square fit to calculate the hydrodynamic diameter and the PolyDispersity Index (PDI). The z-average is an intensity-based overall average hydrodynamic size based on a specific fit to the raw correlation function data. The PDI is a measure of the degree of polydispersity of the sample. Zeta potential measurements were performed which measure the surface charge of particles by measuring their velocity while they are moving due to electrophoresis. Samples were diluted to c.5 mM and analysis was performed at 25°C. All measurements were performed three times, the average with standard deviation was reported.
10
TiO2-NPs Characterization in Cell Medium
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TiO2-NPs with 18 nm core diameter (as provided by manufacturer) were obtained from NanoGrade, Nanocomposix (San Diego, CA) and stock solutions were prepared in complete cell medium and dispersed by sonication according the previously described protocol used by [39 (link)]. NPs were characterized using zeta potential (Z-potential) and dynamic light scattering (DLS) analysis. The measurements were assessed with a Malvern Zetasizer Nano series V5.03 (PSS0012-16 Malvern Instruments, Worcestershire, UK) and the analysis program dispersion technology software (Malvern Instruments, UK). The polydispersity index (PDI) was also assessed. Measurements were conducted in triplicates. Images of TiO2-NPs in cell medium were examined in a Jeol transmission electron microscopy 1230 (TEM) (Japan) at 100 kV.
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