A total of 1 × 106 KYSE30 cells or 3 × 106 KYSE450 cells were subcutaneously injected into 6-week-old male Balb/c nude mice (GemPharmatech Co., Ltd., Nanjing, China). A total of 1 × 106 KYSE150 cells were subcutaneously injected into 6-week-old female Balb/c nude mice (GemPharmatech Co., Ltd., Nanjing, China). The tumor size was measured every other day once the tumor reached 25 mm3. The tumor volume was calculated as 0.5 × length × width2. After the tumors reached 25 mm3, the mice were randomly divided into vehicle or drug groups. MK1775 (60 mg/kg, p.o.) or decitabine (1.0 mg/kg, i.p.) was administered daily or once every two days until sacrifice. Mice were sacrificed 2 weeks after drug administration, and tumors were dissected and weighed. The animal experimental procedures were approved by the Animal Care and Use Committee of the Chinese Academy of Medical Sciences Cancer Hospital.
Balb c nude mice
Manufactured by GemPharmatech
Sourced in China
BALB/c nude mice are a strain of laboratory mice that are athymic, meaning they lack a functional thymus gland and are therefore unable to produce mature T cells. This results in a compromised immune system, making them useful for research involving xenograft models and the study of human diseases and cancer.
Automatically generated - may contain errors
Lab products found in correlation
Matrigel, by Corning (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Lumina lt, by PerkinElmer (2 mentions)
Ivis 100 imaging system, by PerkinElmer (2 mentions)
D luciferin potassium salt, by Abcam (2 mentions)
C57bl 6, by GemPharmatech (2 mentions)
Ivis spectrum in vivo imaging system, by PerkinElmer (2 mentions)
D luciferin potassium salt, by Promega (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
S1060, by Selleck Chemicals (1 mentions)
L calc dilution software, by STEMCELL (1 mentions)
Ivis spectrum imaging system, by PerkinElmer (1 mentions)
Goat anti rabbit igg, by Abcam (1 mentions)
Be0089, by BioXCell (1 mentions)
Be0146, by BioXCell (1 mentions)
C57bl 6 mice, by GemPharmatech (1 mentions)
D000521, by GemPharmatech (1 mentions)
D luciferin, by PerkinElmer (1 mentions)
Ivis lumina series 3, by PerkinElmer (1 mentions)
X rad 225xl biological irradiator, by Rad Source (1 mentions)
153 protocols using balb c nude mice
1
Xenograft Tumor Growth Assay with ESCC Cell Lines
2
In Vivo Xenograft and Zebrafish Metastasis Assays
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The Institutional Animal Care and Use Committee of Xuzhou Medical University provided full approval for this research (IACUC-2202049). BALB/c nude mice were acquired from GemPharmatech, Inc. (Nanjing, China). BALB/c nude mice were obtained from GemPharmatech, Inc. (Nanjing, China). The participants were randomly divided into two groups (n = 5, with no blinding performed in either group). Cells were injected subcutaneously into the axilla of the nude mice, with each mouse receiving a dosage of 1 × 107 cells. The maximum diameter of the tumor was measured every 2 days after implantation. After 12 days, the mice were euthanized, and the tumor was removed and weighed. AB line zebrafish were acquired from China Zebrafish Resource Center (Wuhan, China). They were randomly divided into two groups (n = 16, no blinding was performed, respectively). After A549 cells incubated with CFSE (Thermo Fisher, Waltham, MA, USA), xenograft perivitelline injection were conducted in 2 day-post-fertilization zebrafish via a Picoliter Microinjector (PLI-100A; Warner Instruments, Hollister, MA, USA) with a glass capillary needle (Sutter, Q100-50-10) made on a laser-based needle puller (P-2000; Sutter, Sacramento, CA, USA). Observed cell extravasation in the caudal vasculature at 3 days post-transplant and imaging on a Zeiss AxioZoom V16 fluorescence microscope.
3
Xenograft Mouse Model of SPRYD4 Overexpression
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To establish xenograft mouse models, 1 × 106 HUCCT1 SPRYD4-OV and control group cells were injected subcutaneously into 6-week-old male BALB/c-nude mice (GemPharmatech, China) (n = 6 per group). The tumour volume was measured and recorded using a calliper every 10 days. Fifty days after implantation, the mice were sacrificed by dislocation of cervical vertebra and then the xenografts were removed, weighed, recorded and used in IHC analysis to detect the levels of SPRYD4, Ki67 and Tunel. The Ethics Association of Guangdong Provincial People’s Hospital approved the animal experiments. All 6-week old male BALB/c nude mice were purchased from GemPharmatech (Jiangsu, China).
4
Xenograft Mouse Model of Colorectal Cancer
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All male BALB/c nude mice (4 weeks old) were purchased from GemPharmatech (Nanjing, China). All mice were kept under 12:12 LD conditions with the light on from 8 a.m. to 8 p.m. and fed with water and antibiotic-free food ad libitum. All animal experiments were performed with the approval of the Institutional Animal Care and Use Committee. HCT116 cell suspensions [5 × 106 cells mixed in 200 μL containing serum-free medium and growth reducing Matrigel (1:1)] were subcutaneously injected into the backs of mice. One week after cell inoculation, mice were treated with 5-FU (30 mg kg−1, twice a week) or PBS at the indicated time points for 4 weeks. Tumor weight and volume were monitored once a week. At the endpoint of the experiment, the mice were sacrificed to compare tumor weight and volume.
5
Combination therapy for pancreatic cancer
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The animal experiments were approved by the Institutional Animal Care and Use Committee of Southeast University. PANC-1 cells (1 × 106) in 100 μl PBS were inoculated subcutaneously into 6-week-old female BALB/c nude mice (GemPharmatech Co., Ltd., Nanjing, China). Tumor-bearing mice were randomly grouped when the average tumor volume reached approximately 100 mm3. Mice were administered olaparib (orally, 100 mg/kg) (S1060, Selleck, Houston, USA), PP242 (intraperitoneal (IP) injection, 20 mg/kg) (S2218, Selleck, Houston, USA), or both. Tumor volume was measured every three days. At the endpoint of the experiment, the mice were sacrificed, and tumor tissues were collected and fixed in formalin.
6
Xenograft Mouse Model of GAS5-Overexpressing Lung Cancer
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Four- to six-week-old female BALB/c nude mice were purchased from GemPharmatech (Nanjing, China). A total of 3 × 106 stable GAS5-overexpressing A549 cells or control A549 cells in 60 μL PBS (mixed with 40 μL Matrigel) were injected subcutaneously into the right flank of each mouse using an insulin syringe (KDL, Wenzhou, China). The tumor size was measured by a caliper every 3 days after 6 days of injection, and the tumor volume was calculated using the following formula: 1/2 (length × width2). Mice were humanely sacrificed on Day 21 and tumors were carefully isolated. The harvested tumors were weighed, photographed, and then fixed with formalin for IHC staining or rapidly frozen in liquid nitrogen for RNA and protein assays. To reduce the influence of individual differences on the experimental results, the same species, strain, sex, and age of animals were selected in this study and their weights were similar when measured before the injection of tumor cells. The operators who injected tumor cells into mice were blinded to the group allocation when they operated. All animal experiments were performed in accordance with a protocol approved by the Animal Care and Use Committee of Jinling Hospital.
7
Establishing Liver and Lung Metastasis Models
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BALB/c nude mice for each experimental group were procured from GemPharmatech Co., Ltd. (Nanjing, China). A liver metastasis model was established in 4-week-old BALB/C mice by injecting 1 × 106 cell suspensions, dissolved in 40 μL PBS, into the subcapsular spleen. The mice were euthanized 6 weeks post-injection. Four-week-old BALB/c nude mice were injected intravenously with 2 × 106 cells in 150 μL PBS suspension to establish a lung metastasis model. The mice were sacrificed 6 weeks after tumor cell injection. Excised lungs and livers were fixed in phosphate buffered formalin and then subjected to tomographic examination with HE staining and panoramic scanning. Finally, metastatic nodules in the lung and liver were carefully examined. Experimental procedure in vivo: After 7 days of the injection, the mice were treated intraperitoneally with 2-D08 (4 mg/kg) or DUBs-IN-2 (1 mg/kg) every 2 days for 35 days. The solvents used for 2-D08 were 5% DMSO, 40% PEG300, and 55% saline. The solvents used for DUBs-IN-2 were 50% PEG300 and 50% saline as recommended by the manual.
8
Xenograft Tumor Growth Inhibition
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All animal experiments were approved by the Medical Ethics Committee of Shandong University. Female BALB/c nude mice (5–6 weeks old, 16–18 g) were purchased from GemPharmatech Company (Nanjing, China). Mice were randomly divided into three groups (n = 6/group). Stable clones of QBC939 cells (5 × 106 cells), transfected with shcontrol, shTCF7–1, or shTCF7–2, were subcutaneously injected into the right flanks of nude mice. Tumor diameters were measured with an external caliper every 3 days. Tumor volume was calculated according to the formula V = (L × W2)/2, where V is the volume (mm3), L is the length (mm), and W is the width (mm). Similar methods were used to establish xenografts with FOSL1 knockdown. Wnt agonist 1 (3.5 mg/kg) was injected daily via the tail vein from day 3 after tumor cell injection.
9
In vivo xenograft assay of GLUD1 mutants
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4-week-old male BALB/c-Nude mice were purchased from Gempharmatech Co., Ltd (Jiangsu, China). All mice were lived in the SPF animal facility of the Institute of Translational Medicine at Nanchang University and experimental procedures were approved by the Institutional Animal Use and Care Committee of Nanchang University. All animal experiments were performed according to the established guidelines. The study complied with all the relevant ethical regulations on animal research.
For vivo xenograft assay: A549 cells were transfected with pCDH-Flag-GLUD1 or pCDH-Flag-GLUD1K503R plasmid and constructed stable cell line overexpressing Flag-GLUD1 or Flag-GLUD1K503R. A total volume of 100μl of cell suspensions (1 × 107 cells) were injected subcutaneously into the flanks of nude mice. Four weeks later, the mice were euthanized. Tumors were dissected out and their volumes and weight were measured. Tumor volume was calculated as π/6 × (large diameter) × (smaller diameter).2 (link)
For vivo xenograft assay: A549 cells were transfected with pCDH-Flag-GLUD1 or pCDH-Flag-GLUD1K503R plasmid and constructed stable cell line overexpressing Flag-GLUD1 or Flag-GLUD1K503R. A total volume of 100μl of cell suspensions (1 × 107 cells) were injected subcutaneously into the flanks of nude mice. Four weeks later, the mice were euthanized. Tumors were dissected out and their volumes and weight were measured. Tumor volume was calculated as π/6 × (large diameter) × (smaller diameter).2 (link)
10
Zhongjiefeng and Rutin Suppress 4T1 Tumor Growth
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Briefly, 15 female BALB/C nude mice (3–4 weeks old) (Gempharmatech, Jiangsu, China) were utilized (5 mice/group). The 4T1 cell suspension (100 μL, 5 × 107/mL) was subcutaneously implanted in the right armpit of nude mice. After tumorigenesis (0.5–0.7 cm3), the mice were administered Zhongjiefeng (STATE MEDICAL PERMITMENT No. Z20090321, 5 mg/kg, gavage, once a day) and rutin (500 mg/kg, intraperitoneal injection, once a day). Tumor size was calculated by the following formula: volume (mm3) = length × width2/2. Twenty days later, the tumors were removed after the animals had been euthanized, followed by measurement of the tumor weight and processing of the tumor tissues for subsequent analyses. The study was approved by the Ethics Committee of Guangzhou Forevergen Medical Laboratory Animal Center (approval number IACUC-AEWC-F2303012).
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