Eclipse ni u microscope

Manufactured by National Instruments

The ECLIPSE Ni-U microscope is a high-performance upright microscope designed for a wide range of applications in research and industrial settings. It features a sturdy and ergonomic construction, providing a stable platform for precise observation and imaging. The microscope is equipped with a variety of optical components, including objectives and eyepieces, allowing users to achieve clear and detailed visualization of samples.

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Lab products found in correlation

Discovery xt processor, by Roche (4 mentions) Dab detection kit, by Roche (4 mentions) Alexa fluor 488 or 568 tyramide reagent, by Thermo Fisher Scientific (2 mentions) Fv 1000 filter confocal system, by Olympus (2 mentions) Ds qi2 camera, by National Instruments (2 mentions) Biotinylated goat anti rabbit igg, by Vector Laboratories (2 mentions) Ezprep buffer, by Roche (2 mentions) Cc1 buffer, by Roche (2 mentions) Anti cd3, by Agilent Technologies (1 mentions) Biotinylated horse anti mouse igg, by Vector Laboratories (1 mentions) Anti cd68, by Agilent Technologies (1 mentions) Anti cd4, by Roche (1 mentions) Permount, by Thermo Fisher Scientific (1 mentions) Mab360, by Merck Group (1 mentions) Dako animal research kit for mouse primary antibodies, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Ni-U microscope Eclipse Ni microscope

8 protocols using eclipse ni u microscope

Immunohistochemical Analysis of Pancreatic Cancer Xenografts

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Immunohistochemical analysis of OCT-embedded tumors was performed using a previously-described methodology [34 (link)]. Tissues were stained using 2 µg/ml of T-BsAbs.
22 paraffin-embedded patient-derived xenograft pancreatic cancer tissues were provided by the MSK Antitumor Assessment Core Facility. Tissues were sectioned and immunohistochemical staining was performed by the MSK Molecular Cytology Core Facility as previously described [16 (link)].
All images were captured from prepared slides using a Nikon ECLIPSE Ni-U microscope and NIS-Elements imaging software. EGFR and HER2 staining were analyzed and interpreted by a pathologist using guidelines described by Atkins et al. [35 (link)] and Wolff et al. [36 (link)], respectively.

Long A.W., Xu H., Santich B.H., Guo H., Hoseini S.S., de Stanchina E, & Cheung N.K. (2024). Heterodimerization of T cell engaging bispecific antibodies to enhance specificity against pancreatic ductal adenocarcinoma. Journal of Hematology & Oncology, 17, 20.

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Immunohistochemistry and Immunofluorescence of Tumor Samples

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Immunohistochemistry (IHC) and immunofluorescence (IF) staining were performed at the MSK Molecular Cytology Core Facility using Discovery XT processor (Ventana Medical Systems) as previously described [22 (link)]. Tumor samples were fixed and embedded in paraffin. Anti-human CD45, anti-human CD3, anti-human CD4, anti-human CD8, anti-human FoxP3, anti-mouse CD45, anti-mouse CD11b, anti-mouse CD68, and anti-mouse IBA1 antibodies were used, which were followed by biotinylated secondary antibody. The detection was performed using a DAB detection kit (Ventana Medical Systems) or Alexa Fluor™ 488 or 568 Tyramide Reagent (Invitrogen). IHC images were captured from tumor sections using a Nikon ECLIPSE Ni-U microscope and NIS-Elements 4.0 imaging software. IF images were captured using Leica Inverted Confocal SP8 and processed with Imaris (Bitplane). Antigen positive cells were counted with Qupath 0.1.2.

Park J.A., Wang L, & Cheung N.K. (2021). Modulating tumor infiltrating myeloid cells to enhance bispecific antibody-driven T cell infiltration and anti-tumor response. Journal of Hematology & Oncology, 14, 142.

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Immunohistochemical Profiling of Tumor Immune Microenvironment

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Immunohistochemistry (IHC) staining was performed at the MSK Molecular Cytology Core Facility using Discovery XT processor (Ventana Medical Systems) as previously described.24 25 (link) Tumor samples were fixed and embedded in paraffin. Anti-human CD45 (Abcam Cat# ab10558, RRID:AB_442810), anti-human CD3 (Agilent, Cat# A0452, RRID:AB_2335677), anti-human CD4 (Ventana Medical Systems Cat# 790-4423, RRID:AB_2335982), anti-human CD8 (Ventana Medical Systems Cat# 790-4460, RRID:AB_2335985), and anti-mouse CD31(Abcam Cat# ab134168, RRID:AB_2890012) were used, followed by a biotinylated secondary antibody. The detection was performed using a DAB detection kit (Ventana Medical Systems) or Alexa Fluor 488 or 568 Tyramide Reagent (Invitrogen). IHC staining of peripheral node addressin (PNA) (MECA79) was done with a manual protocol at Laboratory of Comparative Pathology using anti-PNA antibody (MECA79R) (Novus Cat# NBP2-78792). Immunofluorescence (IF) double staining of PNA and human CD3 was performed with the identical anti-PNA antibody and monoclonal rabbit anti-human CD3 antibody (clone MRQ-39, LS-C202826). Microscopic images were captured from tumor sections using a Nikon ECLIPSE Ni-U microscope and NIS-Elements V.4.0 imaging software. Antigen positive cells were counted with Qupath V.0.1.2 or using positive pixel count analysis.

Park J.A., Espinosa-Cotton M., Guo H.F., Monette S, & Cheung N.K. (2023). Targeting tumor vasculature to improve antitumor activity of T cells armed ex vivo with T cell engaging bispecific antibody. Journal for Immunotherapy of Cancer, 11(3), e006680.

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Widefield and Confocal Microscopy Techniques

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Widefield micrographs were collected using a Nikon Eclipse Ni-U microscope with a DS-Qi2 camera and NIS-Elements Advanced Research software. Confocal micrographs were collected using the Olympus FV 1000 Filter Confocal system in the Campus Microscopy and Imaging Facility at the Ohio State University.

Pécot T., Cuitiño M.C., Johnson R.H., Timmers C, & Leone G. (2022). Deep learning tools and modeling to estimate the temporal expression of cell cycle proteins from 2D still images. PLoS Computational Biology, 18(3), e1009949.

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Immunohistochemical Profiling of Tumor Immune Cells

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The immunohistochemical (IHC) detection was performed at the MSK Molecular Cytology Core Facility using Discovery XT processor (Ventana Medical Systems). Paraffin-embedded tumor sections were deparaffinized with EZPrep buffer (Ventana Medical Systems), antigen retrieval was performed with CC1 buffer (Ventana Medical Systems) and sections were blocked for 30 minutes with Background Buster solution (Innovex). Anti-CD3 (DAKO, cat# A0452, 1.2 ug/ml), anti-CD4 (Ventana, cat#790-4423, 0.25ug/ml), CD8 (Ventana, cat#790-4460, 0.35ug/ml) and anti-CD68 (DAKO, cat#M0814, 0.02ug/ml) antibodies were applied and sections were incubated for 5 h, followed by 60 minutes incubation with biotinylated goat anti-rabbit IgG (Vector labs, cat#PK6101) for CD3, CD4 and CD8 antibodies or biotinylated horse anti-mouse IgG (Vector Labs, cat# MKB-22258) for CD68 antibodies at 1:200 dilution. The detection was performed with DAB detection kit (Ventana Medical Systems) according to manufacturer’s instruction. Slides were counterstained with hematoxylin and coverslipped with Permount (Fisher Scientific). Images were captured from tumor sections using Nikon ECLIPSE Ni-U microscope and NIS-Elements 4.0 imaging software, and counts of IHC-positive cells were averaged from two randomly selected fields (200X magnifications).

Xu H., Cheng M., Guo H., Chen Y., Huse M, & Cheung N.K. (2014). Retargeting T cells to GD2 pentasaccharide on human tumors using bispecific humanized antibody. Cancer immunology research, 3(3), 266-277.

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Immunohistochemical Analysis of Xenograft Tumors

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Harvested xenografts were formalin-fixed paraffin-embedded (FFPE) and tested for immunohistochemistry (IHC). IHC staining was performed by Molecular Cytology Core Facility of MSKCC using Discovery XT processor (Ventana Medical Systems). FFPE tumor sections were deparaffinized with EZPrep buffer (Ventana Medical Systems), antigen retrieval was performed with CC1 buffer (Ventana Medical Systems), and sections were blocked for 30 min with background buffer solution (Innovex). Anti-CD3 antibody (Agilent, Cat# A0452, RRID: AB_2335677, 1.2 µg/mL) and anti-HER2 (Enzo Life Sciences Cat#ALX-810-227 L001, RRID: AB_11180914, 5 µg/mL) were applied, and sections were incubated for 5 hours, followed by 60 min incubation with biotinylated goat anti-rabbit IgG (Vector laboratories, cat# PK6101) at 1:200 dilution. The detection was performed with DAB detection kit (Ventana Medical Systems) according to manufacturer’s instruction. All images were captured from tumor sections using Nikon ECLIPSE Ni-U microscope and NIS-Elements V.4.0 imaging software.

Park J.A, & Cheung N.K. (2022). Overcoming tumor heterogeneity by ex vivo arming of T cells using multiple bispecific antibodies. Journal for Immunotherapy of Cancer, 10(1), e003771.

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Widefield and Confocal Microscopy Protocol

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Pécot T., Cuitiño M.C., Johnson R.H., Timmers C., & Leone G. (2021). Deep learning tools and modeling to estimate the temporal expression of cell cycle proteins from 2D still images.

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Histological and Immunohistochemical Analysis

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Tissues were fixed in formalin and paraffin embedded. Multiple sections (4 μm thickness) were deparaffinized with xylene and stained with hematoxylin and eosin (H&E), Masson's trichrome or oil red. Immunohistochemistry was carried out on the same sections, using the following primary antibodies: glial fibrillary acidic protein or anti-GFAP (Millipore, MAB360). Dako Animal
Research Kit for mouse primary antibodies (Dako Diagnóstico S.A., Spain) was used for the qualitative identification of antigens by light microscopy. Sections were examined at 40-400 magnifications with a Nikon Eclipse Ni-U microscope, and the images were scanned under equal light conditions with the NIS-Elements Br computer software.

Hidalgo‐Gutiérrez A., Barriocanal‐Casado E., Diaz‐Casado M.E., González-García P., Chiozzi R.Z., Acuña‐Castroviejo D., & López L.C. (2021). β-RA targets mitochondrial metabolism and adipogenesis, leading to therapeutic benefits against CoQ deficiency and age-related overweight.

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