Polyimide (PI) thin films with a thickness of 50 μm (Kapton® HN Film) were purchased from CS Hyde company. Nickel (RTM Kit) and gold (Immersion Gold CF) electroless plating precursors were purchased from Transene company. Silicone polymers, including Ecoflex 00–50 and Sylagrd 184, were purchased from Smooth-On and Dow Corning. Heptane to dilute Ecoflex, polyvinylpyrrolidone (PVP) for surface treatment, and polyaniline (PANI) for the pH measurement was purchased from Sigma-Aldrich. All chemicals were used without further purification. The cell culture media, including DMEM (11965092) and RPMI (11875119), were purchased from Thermo Fisher Scientific. Another customized cell culture medium composed of salts, amino acids, glucose, and cell-secreted factors, L7, was used to culture human pluripotent stem cells for 24 hours before the measurement.
Dmem 11965092
Manufactured by Thermo Fisher Scientific
Sourced in United States
DMEM (11965092) is a cell culture medium used for the in vitro maintenance and growth of various cell types. It provides essential nutrients, vitamins, and salts required for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylpyrrolidone pvp, by Merck Group (1 mentions)
Polyaniline pani, by Merck Group (1 mentions)
Ecoflex 00 50, by Smooth-On (1 mentions)
Lipo3000, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Normocin ant nr 1, by InvivoGen (1 mentions)
Lncap clone fgc, by American Type Culture Collection (1 mentions)
4 protocols using dmem 11965092
1
Polyimide Thin Films with Electroless Plating
2
Cell Line Authentication and Culture
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The cell lines involved in this study have been performed short tandem repeat (STR) profiling to guarantee authenticity. Moreover, all the cell lines have been tested for mycoplasma negative based on PCR analysis (C0301S, Beyotime Biotechnology, Shanghai, China). Cells were cultured in DMEM (11,965,092, Thermo Fisher Scientific, Inc, CA, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin. Cells were maintained at 37 °C and 5% CO2. Plasmids were transfected through Lipo3000 (L3000150, Thermo Fisher Scientific, Inc.) based on the instructions.
3
Cell Culture Protocols for Various Cell Lines
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Human HEK293T cells (obtained from ATCC) and human GCs of the KGN line (obtained from BIUEFBIO) were grown in Dulbecco’s minimal Eagle medium (DMEM, 11,965-092; Gibco, Grand Island, NY, USA), the human prostate cancer cell line (LNCaP clone FGC, obtained from ATCC) was cultured in RPMI-1640 (61870036; Gibco), and mouse GCs of the GRM02 line (obtained from BIUEFBIO) were maintained in DMEM/F12 (1:1) medium (L310KJ; BasalMedia, Shanghai, China) supplemented with 10% (vol/vol) foetal bovine serum (FBS, A3161002C; Gibco), 1% (vol/vol) penicillin/streptomycin (15140-122; Gibco) and 2% normocin (ant-nr-1; InvivoGen, San Diego, CA, USA) at 37°C in 5% CO2 with 100% humidity.
4
Isolation and Culture of Chicken Spermatogonial Stem Cells
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Primary chicken SSCs were harvested as previously described (Ozturk et al., 2008 (link)). Briefly, the synovial layer sheath of metatarsal extensor tendon was harvested under sterile conditions. Tissues were rinsed with 0.1 mol sterile PBS (pH 7.2), cut into small pieces of 1 mm3, and digested with 0.1% trypsin in PBS for 30 min. Then the mixture was centrifuged. The pellets were washed with PBS and digested in 0.1% collagenase in Dulbecco's modified Eagle medium (DMEM , 11965-092; GIBCO, Waltham, MA) containing 10% fetal bovine serum (FBS , 10099-141; GIBCO, Waltham, MA), 100 U/mL penicillin, and 100 mg/mL streptomycin for 2 h at 37°C. After the digestion process, cells were filtered through a 70-μm nylon sterile filter, washed twice with DMEM, and cultured in DMEM with 10% FBS at 37°C with 5% CO2. The third-to-sixth generation cells were used for experimental research. The chicken HD-11 macrophage cell line was kindly provided by Professor Hongjie Fan at the College of Veterinary Medicine, Nanjing Agricultural University. HD11 cells were cultured in DMEM with 10% FBS at 37°C with 5% CO2.
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