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Clax software

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CLAX software is a data acquisition and analysis tool developed by Columbus Instruments. It is designed to interface with various laboratory equipment and instruments, allowing users to capture, store, and analyze data from their experiments.

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Lab products found in correlation

Oxymax open circuit calorimeter system, by Columbus Instruments (4 mentions) Comprehensive lab animal monitoring system clams, by Columbus Instruments (1 mentions) Clams system, by Columbus Instruments (1 mentions) Oxymax system, by Columbus Instruments (1 mentions) Comprehensive laboratory animal monitoring system clams, by Columbus Instruments (1 mentions) Oxymax, by Columbus Instruments (1 mentions)

9 protocols using clax software

1

Whole Body Composition and Metabolism

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To assess fat vs. lean mass, whole composition analysis was performed using the EchoMRI3in1™ machine from Echo Medical System according to manufacturer’s instructions. To assess any changes in metabolism, we used the Comprehensive Lab Animal Monitoring System (CLAMS) made by Columbus Instruments, Inc. In brief, mice were single-housed in a large enclosure with precise control over the temperature and light / dark cycle. All values are normalized to body weight. Data were analyzed using the CLAX software from Columbus Instruments, exported into Excel and plotted in GraphPad.
Hilgendorf K.I., Johnson C.T., Mezger A., Rice S.L., Norris A.M., Demeter J., Greenleaf W.J., Reiter J.F., Kopinke D, & Jackson P.K. (2019). Omega-3 fatty acids activate ciliary FFAR4 to control adipogenesis. Cell, 179(6), 1289-1305.e21.
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2

Indirect Calorimetry of Metabolic Rates

Cited 2 times
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To measure metabolic rates, metabolic parameters in the treated mice were analyzed with indirect calorimetry43 (link), 44 (link) using the Comprehensive Laboratory Animal Monitoring System (CLAMS) system (Columbus Instruments, Columbus, OH, USA) at normal temperature (22℃). The mice were individually housed 2–3 days before the experiment for habituation and then fed the same diet (chow or HFD) and water provided ad libitum in clear respiratory chambers (20.5 × 10.5 × 12.5 cm). O2 consumption, CO2 production, energy expenditure, heat, and activities of the experimental animals were measured for 24 h. These data were collected with Oxymax for Windows (version 5.40.14, Columbus Instruments) and analyzed with CLAX software (version 2.2.15, Columbus Instruments). Based on the collected data, the respiratory quotient or exchange ratio (CO2/O2) and delta-heat values were calculated using the CLAX software.
Kim D., Lee Y., Kim H.R., Park Y.J., Hwang H., Rhim H., Kang T., Choi C.W., Lee B, & Kim M.S. (2021). Hypothalamic administration of sargahydroquinoic acid elevates peripheral thermogenic signaling and ameliorates high fat diet-induced obesity through the sympathetic nervous system. Scientific Reports, 11, 21315.
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3

Calorimetry and Treadmill Exercise in Mice

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Calorimetry was performed using the Oxymax system from Columbus Instruments (Columbus, OH) as previously described (Powers et al. 2013 (link)). Calorimetry was evaluated for 9h during the initial light phase, 10 h for the dark phase and 4 h during the morning light phase. Resting oxygen consumption and carbon dioxide production rates (VO2 and VCO2 respectively) were measured every 10 minutes and normalized to body weight. Animals were given free access to water and food during testing. Food was weighed following testing. A total of 8 Crt+/y and 10 Crt−/y mice were used.
The following day, mice were exercised on a motorized treadmill placed inside of a sealed chamber. The initial speed of the treadmill was 5 m/min and was increased stepwise by 5 m/min every 5 min until a final speed of 25 m/min was reached. VO2 and VCO2 were sampled every 30 sec and normalized to the body weight of the animal. Respiratory exchange ratio (RER), a measure that determines if carbohydrates, fatty acids, or protein are being utilized for energy (Simonson and DeFronzo 1990 (link)), and energy expenditure were calculated using CLAX software from Columbus Instruments.
Perna M.K., Kokenge A.N., Miles K.N., Udobi K.C., Clark J.F., Pyne-Geithman G.J., Khuchua Z, & Skelton M.R. (2016). Creatine transporter deficiency leads to increased whole-body and cellular metabolism. Amino acids, 48(8), 2057-2065.
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4

Metabolic Profiling of Ddr1 Knockout Mice

Cited 1 time
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Analysis of metabolic parameters was performed in vivo as previously described using the Comprehensive Laboratory Animal Monitoring System (CLAMS; Columbus Instruments, Columbus, OH) [27 (link)]. Energy expenditure, food intake, oxygen consumption (VO2), carbon dioxide production (VCO2), respiratory exchange ratio (RER), and locomotor activity were assessed in Ddr1+/+ and Ddr1−/− mice fed an HFD for 6 weeks (6wk HFD). Mice were acclimatized in the metabolic chambers for 24 h prior to the start of data collection, followed by a 24-hour period of data collection. Data was categorized as diurnal (light cycle) and nocturnal (dark cycle). Data was analyzed using CLAX Software (Columbus Instruments).
Lino M., Ngai D., Liu A., Mohabeer A., Harper C., Caruso L.L., Schroer S.A., Fu F., McKee T., Giacca A., Woo M, & Bendeck M.P. (2020). Discoidin domain receptor 1-deletion ameliorates fibrosis and promotes adipose tissue beiging, brown fat activity, and increased metabolic rate in a mouse model of cardiometabolic disease. Molecular Metabolism, 39, 101006.
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5

Metabolic Flexibility Assessment in Mice

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Indirect calorimetry was performed during light and dark cycles to determine the extent to which treatment affected metabolic parameters during conditions of rest (light cycle) and activity (dark cycle). In addition, the effects of treatment on metabolic flexibility were assessed by measuring metabolic parameters under fed conditions (provision of a predominantly carbohydrate-based fuel source) and then fasted conditions (reliance on endogenous lipid-based energy stores). On the day of the experiment, mice were weighed and acclimated overnight. In a subset of eight mice per group, the habitual ambulatory, rearing, and total activity, oxygen consumption (VO2), and carbon dioxide production (VCO2) of individual mice were monitored over a 24h period (12h light/12h dark) using a Comprehensive Laboratory Animal Monitoring System (CLAMS) equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments). The VO2 and VCO2 values were used to calculate the respiratory exchange ratio (RER) and VO2. RER values were used to determine the basal metabolic rate (in kilocalories per kilogram per hour). Data were analyzed using the CLAX software from Columbus Instruments, exported into Excel and plotted in GraphPad.
Zhang Y., Hao J., Tarrago M.G., Warner G.M., Giorgadze N., Wei Q., Huang Y., He K., Chen C., Peclat T.R., White T.A., Ling K., Tchkonia T., Kirkland J.L., Chini E.N, & Hu J. (2021). FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue. Cell reports, 36(5), 109481.
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6

Indirect Calorimetry Monitoring of Murine Metabolism

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Indirect calorimetry was performed during light and dark cycles to determine the extent to which treatment affected metabolic parameters during conditions of rest (light cycle) and activity (dark cycle). On the day of the experiment, mice were weighed and acclimated overnight. In a subset of eight mice per group, the habitual ambulatory, rearing, and total activity, oxygen consumption (VO2), and carbon dioxide production (VCO2) of individual mice were monitored over a 24 h period (12 h light/12 h dark) using a Comprehensive Laboratory Animal Monitoring System (CLAMS) equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments). The VO2 and VCO2 values were used to calculate the respiratory exchange ratio (RER) and VO2. RER values were used to determine the basal metabolic rate (in kilocalories per kilogram per hour). Data were analyzed using the CLAX software from Columbus Instruments, exported into Excel and plotted in GraphPad.
Burr S.D., Chen Y., Hartley C.P., Zhao X, & Liu J. (2023). Replacement of saturated fatty acids with linoleic acid in western diet attenuates atherosclerosis in a mouse model with inducible ablation of hepatic LDL receptor. Scientific Reports, 13, 16832.
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7

Whole-body Energy Metabolism Monitoring

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Whole-body energy metabolism measurements were performed using a Comprehensive Laboratory Animal Monitoring System (CLAMS) equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments, Columbus, OH, USA). Mouse weights were measured and mice were acclimated for 3 days in a metabolic chamber with food and water under a normal 12-hours light-dark cycle. Oxygen consumption (VO2) and carbon dioxide production (VCO2) were monitored for 24 hours. The VO2 and VCO2 values were used to calculate the respiratory exchange ratio (RER). RER was used to assess energy source utilization and energy expenditure (EE) normalized by body weight. Data were analyzed using the CLAX software from Columbus Instruments and visualized using an Excel.
Takaoka S., Yanagiya A., Mohamed H.M., Higa R., Abe T., Inoue K.I., Takahashi A., Stoney P, & Yamamoto T. (2021). Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis. iScience, 24(10), 103151.
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8

Comprehensive Metabolic Profiling of Mice

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Daily activity, food and water intake, metabolic performance, temperature were measured for the 23 months old PL and PLD mice on a CLAMS system equipped with Oxymax open-circuit calorimeter (Columbus Instruments, Columbus, OH). Physiological and behavioral parameters for individual mouse were monitored over a 24 h period (12 h light/dark cycle), and results were analyzed by CLAX software (Columbus Instruments).
Wang B., Wang L., Gasek N.S., Zhou Y., Kim T., Guo C., Jellison E.R., Haynes L., Yadav S., Tchkonia T., Kuchel G.A., Kirkland J.L, & Xu M. (2021). An inducible p21-Cre mouse model to monitor and manipulate p21-highly-expressing senescent cells in vivo. Nature aging, 1(10), 962-973.
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9

Metabolic Flexibility Assessment in Mice

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Indirect calorimetry was performed during light and dark cycles to determine the extent to which treatment affected metabolic parameters during conditions of rest (light cycle) and activity (dark cycle). In addition, the effects of treatment on metabolic flexibility were assessed by measuring metabolic parameters under fed conditions (provision of a predominantly carbohydrate-based fuel source) and then fasted conditions (reliance on endogenous lipid-based energy stores). On the day of the experiment, mice were weighed and acclimated overnight. In a subset of eight mice per group, the habitual ambulatory, rearing, and total activity, oxygen consumption (VO2), and carbon dioxide production (VCO2) of individual mice were monitored over a 24h period (12h light/12h dark) using a Comprehensive Laboratory Animal Monitoring System (CLAMS) equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments). The VO2 and VCO2 values were used to calculate the respiratory exchange ratio (RER) and VO2. RER values were used to determine the basal metabolic rate (in kilocalories per kilogram per hour). Data were analyzed using the CLAX software from Columbus Instruments, exported into Excel and plotted in GraphPad.
Zhang Y., Hao J., Tarrago M.G., Warner G.M., Giorgadze N., Wei Q., Huang Y., He K., Chen C., Peclat T.R., White T.A., Ling K., Tchkonia T., Kirkland J.L., Chini E.N, & Hu J. (2021). FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue. Cell reports, 36(5), 109481.
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