Uas p35

Manufactured by Bloomington Drosophila Stock Center

Sourced in United Kingdom

The UAS-p35 is a genetic tool used in Drosophila research. It contains the p35 gene, which encodes an anti-apoptotic protein that inhibits programmed cell death. This tool can be used to prevent cell death in specific tissues or cell types when expressed in a controlled manner.

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UAS-p35 (III), (2 citations)

29 protocols using uas p35

Drosophila Genetics: Transgenic Manipulation

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All the fly stocks were raised on standard Drosophila corn media and crosses were performed at 25°C unless otherwise indicated. UAS-APLP2 was kindly provided by Dr. Merders; puc^H246, UAS-puc-IR, UAS-mmp1-IR, and UAS-p35 were obtained from Bloomington Stock Center; UAS-Bsk^DN, UAS-puc[26 (link)], ptc-Gal4, en-Gal4, puc^E69[29 (link)], and UAS-LacZ [30 (link)] were previously described.

Wang X., Guo X., Ma Y., Wu C., Li W, & Xue L. (2018). APLP2 Modulates JNK-Dependent Cell Migration in Drosophila. BioMed Research International, 2018, 7469714.

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Drosophila Larval Genotyping and Staging

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The following stocks were obtained from the Bloomington Drosophila Stock Center: yw (used as the WT in this study; 1495), HistoneH2Av:RFP (23651), sas-4^s2214 (12119), 1407-Gal4 (=insc-Gal4; 8751), UAS-p35 (5072), cnn^HK21 (5039), Df(3L)BSC438 (24942), UAS-bsk^DN (6409), UAS-p53^H159N (8420), and Df(2R)w45-30n cn¹ (deletes mad1; 4966). Additional fly stocks used were mad2^P (Buffin et al., 2007 (link)), mad1¹ (from R. Karess [Emre et al., 2011 (link)] via D. Fox [Stormo and Fox, 2016 (link)]), asl^mecD (Blachon et al., 2008 (link)), MoesinFABD:GFP (a gift from D. Kiehart, Duke University, Durham, NC), TRE-GFP (Chatterjee and Bohmann, 2012 (link)), BubR1:GFP (Buffin et al., 2005 (link)), and Hid>GFP (Tanaka-Matakatsu et al., 2009 (link)). Mutant genotypes were balanced by TM6b Tb Hu stock, which allowed selection of non-Tb homozygous third instar larvae by body shape. When third chromosome mutations were in combination with second chromosome mutations or transgenes, a fused Cyo:TM6b balancer was used. Homozygous embryos and second instar larvae were selected using TM3,Ser,act-GFP, allowing selection against GFP. All stocks and crosses were maintained at 25°C. Late third instar larvae were collected 6 d AEL, mid–third instar larvae were collected 4 d AEL, and second instar larvae were collected 2 d AEL.

Poulton J.S., Cuningham J.C, & Peifer M. (2017). Centrosome and spindle assembly checkpoint loss leads to neural apoptosis and reduced brain size. The Journal of Cell Biology, 216(5), 1255-1265.

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Drosophila Genetic Manipulation Protocol

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Fly stocks were maintained at 25 °C on standard food containing 0.7% agar, 5% glucose, and 7% dry yeast. Canton S was used as the wt strain. Flies carrying w; Sp/CyO; UAS-hsrω RNAi (Bloomington, 59616) and + ;UAS-93D (Bloomington, 59614) were described previously^{32 (link),43 (link)}. Flies carrying + ;UAS-GFP were obtained from the Kyoto stock center (DGRC 107870). Flies carrying UAS-GFP IR;+ (41550), + ;UAS-DIAP1 (6657), + ;UAS-P35 (5073), LAMP1^MI15602/Cyo;+ (59738), and Sp/Cyo;UAS-HRP-LAMP1 (42701) were obtained from the Bloomington stock center. The fly line GMR-GAL4 was described previously^{57 (link)}. The fly stock carrying the hFUS transgene was described previously³⁴ and the Atg8a loss-of-function mutant fly was a gift from Juhász G.^{46 (link)}.

Lo Piccolo L., Jantrapirom S., Nagai Y, & Yamaguchi M. (2017). FUS toxicity is rescued by the modulation of lncRNA hsrω expression in Drosophila melanogaster. Scientific Reports, 7, 15660.

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Drosophila Genetics Protocols Database

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Flies were grown on a molasses-based food at 25 °C.
The following Drosophila stocks were used: (i) w¹¹¹⁸; FRT82B, (ii) Df(3R)Exel6169,P{XP-U}Exel6169/TM6B,Tb (#7648), (iii) UAS-RasV12, FRT82B (RAS^High—modified from stock #4847), (iv) FRT82B, UAS-P35, (v) UAS-Ampk^Trip20(RNAi) (#57785)—all provided by the Bloomington Drosophila Stock Center. UAS-RasV12; FRT82B (RAS^Low)^{14 (link)} was provided by Tian Xu. w¹¹¹⁸ and Viking-GFP^{51 (link)} were gifts from K. Moberg (Emory University). Lkb1^4A4-2 and Lkb1^4B1-11 were gifts from J. McDonald (Kansas State University). Lkb1^X5 was a gift from W. Du (University of Chicago). Fluorescently labeled mitotic clones were induced in larval eye-imaginal discs using the following strain: y,w, eyFLP1; Act >y+ >Gal4, UAS-GFP (or RFP); FRT82B, Tub-Gal80 (provided by Tian Xu).

Rackley B., Seong C.S., Kiely E., Parker R.E., Rupji M., Dwivedi B., Heddleston J.M., Giang W., Anthony N., Chew T.L, & Gilbert-Ross M. (2021). The level of oncogenic Ras determines the malignant transformation of Lkb1 mutant tissue in vivo. Communications Biology, 4, 142.

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Drosophila Husbandry and Genetic Stocks

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Flies were raised on standard cornmeal-molasses-agar medium. Female progeny from experimental crosses were collected and maintained with less than 30 flies per vial. Flies were turned onto fresh food vials every two days. The following fly stocks used were from the Bloomington Drosophila Stock Center (BDSC), Vienna Drosophila Stock Center (VDRC), or generous gifts from the fly community as indicated: Gal80^ts; UAS-lacZ^NLS; UAS-p35 (Bloomington stock center #7018, #3956 and #5073); esgGal4, UASGFP (gift from Norbert Perrimon); esgGal4,2xYFP; Su(H)Gal80, tub-Gal80ts (gift from Steven Hou) hdcRNAi lines used were from Vienna Drosophila stock center and labeled as UAS-HdcRNAi¹ (VDRC#45069) and UAS-HdcRNAi² (VDRC#104322). Wild-type flies were Oregon R. More detailed information about these stocks can be found at Flybase (http://flybase.bio.indiana.edu).

Resende L.P., Truong M.E., Gomez A, & Jones D.L. (2017). Intestinal stem cell ablation reveals differential requirements for survival in response to chemical challenge. Developmental biology, 424(1), 10-17.

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Drosophila Genetic Manipulations

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All stocks were raised on standard Drosophila media and crosses were performed at 25°C unless otherwise indicated. UAS-APLP1, UAS-APPL^sd were kindly provided by Dr. Merdes [22 (link)]; puc^H246, UAS-mmp1-IR, UAS-mmp2-IR, UAS-TIMP1, UAS-Ras^V12, UAS-Rho1-IR, UAS-p35, UAs-P53^DN were obtained from Bloomington Stock Center; sd-Gal4, UAS-Bsk^DN, UAS-Puc, UAS-Egr, UAS-Hep [70 (link)]; ptc-Gal4, en-Gal4, puc^{E69 34}, UAS-LacZ [71 (link)] were previously described.

Wang X., Sun Y., Han S., Wu C., Ma Y., Zhao Y., Shao Y., Chen Y., Kong L., Li W., Zhang F, & Xue L. (2017). Amyloid precursor like protein-1 promotes JNK-mediated cell migration in Drosophila. Oncotarget, 8(30), 49725-49734.

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Drosophila Genetics Protocols for Research

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All flies were raised on standard food at 25 °C. UAS-(GR)₈₀ and UAS-CONT-(GR)₈₀ flies as well as UAS-(G₄C₂)₁₆₀ and UAS-(G₄C₂)₅ flies have been obtained from earlier studies^{40 (link)}. A deficiency kit for the second chromosome and ELAV-GAL4, TubGAL80^ts, UAS-p35, lilli^k05431, lilli^A17-2, UAS-GFP, UAS-lacZ, OK³⁷¹-GAL4, and GMR-GAL4 fly stocks were from the Bloomington Drosophila Stock Center. Lilli RNAi line (v106142) was from the Vienna Drosophila Resource Center (VDRC). lilli^4U5 and UAS-lilli-HA fly stocks were a gift from Dr Ernst Hafen. The lilli^A17-2 allele was created by ethyl methanesulfonate mutagenesis. The nature of the mutation is unknown but is thought to be a loss of function allele^{55 (link)}. The lilli^k05431 allele is a P-element insertion^{56 (link)}. The lilli^4U5 allele has a short deletion resulting in a frameshift and a functional null allele^{56 (link)}.

Yuva-Aydemir Y., Almeida S., Krishnan G., Gendron T.F, & Gao F.B. (2019). Transcription elongation factor AFF2/FMR2 regulates expression of expanded GGGGCC repeat-containing C9ORF72 allele in ALS/FTD. Nature Communications, 10, 5466.

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Genetic Manipulation Techniques in Drosophila

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Fly stocks were maintained at 25 °C on standard food containing 0.65% agar, 10% glucose, 4% dry yeast, and 5% cornmeal. Transgenic flies with UAS-dSerT-IR_686–1079 (SerT-IR1) and UAS-dSerT-IR_1740–1760 (SerT-IR2) were obtained from the Vienna Drosophila Resource Center (#100584; Vienna, Austria) and Bloomington Drosophila Stock Center (#62985; Bloomington, IN, USA), respectively. These flies carried an IR of the SerT gene (targeting regions from nucleotide 686 to 1079 and from 1740 to 1760, respectively) downstream of the UAS sequence, on the second chromosome. All other flies used in this study were obtained from Bloomington Drosophila Stock Center: UAS-gfp (#1522), UAS-p35 (#5072), UAS-diap1 (#6657), UAS-PI3K (#8287). yw flies were used as the wild-type strain.

Pham T.L., Binh T.D., Liu G., Nguyen T.Q., Nguyen Y.D., Sahashi R., Men T.T, & Kamei K. (2020). Role of Serotonin Transporter in Eye Development of Drosophila melanogaster. International Journal of Molecular Sciences, 21(11), 4086.

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Overexpression of Salr, Salm, and SALL4 in Drosophila

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Fly lines were cultured at 25°C on standard fly food unless otherwise noted. The transgenes used were as follows: UAS-salr (de Celis et al., 1996 (link)), UAS-salm (from the Bloomington Drosophila Stock Center #29716, short for BL#29716), UAS-SALL4-HA (BL#65835), UAS-Timp (BL#58708), UAS-bsk^DN (Weber et al., 2000 (link)), UAS-p35 (BL#5073), UAS-Diap1 (BL#6657), UAS-GFP (nuclear expression, BL#4775), UAS-CD8-GFP (membrane expression) (Lee and Luo, 1999 (link)), UAS-dMyc (BL#9674), dMyc-RNAi (BL#36123), puc-lacZ (Martin-Blanco et al., 1998 (link)), UAS-puc (Dobens et al., 2001 (link)), dpp-Gal4 (Shen and Mardon, 1997 (link)), actin5c>CD2>Gal4 (Pignoni and Zipursky, 1997 (link)), and AB1-Gal4 (BL#1824). To promote the GFP phenotype in a larval body, salm, salr, or SALL4-overexpressing larvae were raised at 29°C after egg laying. Clones in the larval wing imaginal discs were generated with the genotypes y w¹¹¹⁸ hs-Flp; actin5c>CD2>Gal4 UAS-GFP/CyO; UAS-salr/UAS-SALL4-HA by heat shock at 35.5°C for 30 min. Then, late third-instar larvae were dissected after a recovery period of 3 days at 25°C.

Sun J., Zhang J., Wang D, & Shen J. (2020). The transcription factor Spalt and human homologue SALL4 induce cell invasion via the dMyc-JNK pathway in Drosophila. Biology Open, 9(3), bio048850.

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Drosophila Model for Alzheimer's Disease

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All flies were raised on standard cornmeal/molasses/agar media at 25°C. Experimental genotypes were generated using the Gal4/UAS system for expressing specific proteins in a temporal and tissue specific manner, as previously described (Brand and Perrimon, 1993 (link)). All crosses were performed using engrailed:Gal4 (Brower, 1986 (link)), which expresses the Gal4 protein specifically within the posterior compartment of Drosophila wings. The anterior compartment served as an internal control for each experiment. Transgenic Drosophila genotypes used were: UAS:APP (Fossgreen et al., 1998 (link)), UAS:APP and UAS:BACE (Greeve et al., 2004 (link)), UAS:p35 (Bloomington Stock Center # 5072), UAS:ubiquilin RNAi (Vienna Drosophila RNAi Center Transformant ID#s 47448 and 47449). UAS:GFP (Bloomington Stock Center # 4775) was used as the wild type control.

Zhang C., Inamdar S.M., Swaminathan S., Marenda D.R, & Saunders A.J. (2022). Association of the Protein-Quality-Control Protein Ubiquilin-1 With Alzheimer’s Disease Both in vitro and in vivo. Frontiers in Neuroscience, 16, 821059.

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