Formalin solution

Manufactured by CellPath

10% formalin solution is a fixative used in histology and pathology laboratories. It is a dilute solution of formaldehyde in water. The primary function of this solution is to preserve and fix tissue samples for further analysis and processing.

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Lab products found in correlation

Dm4000b light microscope, by Leica (1 mentions) Safranin o, by Merck Group (1 mentions) Masson s trichrome, by Merck Group (1 mentions) Hematoxylin and eosin, by Thermo Fisher Scientific (1 mentions)

2 protocols using formalin solution

Histological Analysis of Day 7 Cartilage

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Due to their larger size, histology was performed only on the day 7 cartilage discs. One half of a disc was sectioned, fixed in 10% formalin solution (CellPath) overnight and wax embedded following standard processing protocols. Four μm transverse sections were taken, with one section from each treatment mounted onto each Cellstik Superfrost Ultra Plus slide (CellPath). Slides were stained with haematoxylin and eosin (Licor), Safranin-O (Sigma) and Masson’s Trichrome (Sigma) and images were taken using a Leica DM4000B light microscope at 20 x magnification.

Ratnayake M., Tselepi M., Bloxham R., Plöger F., Reynard L.N, & Loughlin J. (2017). A consistent and potentially exploitable response during chondrogenesis of mesenchymal stem cells from osteoarthritis patients to the protein encoded by the susceptibility gene GDF5. PLoS ONE, 12(5), e0176523.

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Tissue Fixation and Staining Protocol

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Tissues were fixed in a 10% formalin solution (CellPath Ltd.) and subsequently embedded in paraffin blocks. Bones were decalcified using 0.38 M EDTA (pH7) solution. Tissue sections (4 µm) were stained with Hematoxylin and Eosin (Thermo Fisher Scientific) according to the manufacturer’s protocol. Peripheral blood (PB) and BM cytopsin preparations were fixed in methanol and subsequently stained with Eosin Y and Methylene blue (Rapid Romanowsky Stain; TCS Biosciences Ltd.).

Giotopoulos G., van der Weyden L., Osaki H., Rust A.G., Gallipoli P., Meduri E., Horton S.J., Chan W.I., Foster D., Prinjha R.K., Pimanda J.E., Tenen D.G., Vassiliou G.S., Koschmieder S., Adams D.J, & Huntly B.J. (2015). A novel mouse model identifies cooperating mutations and therapeutic targets critical for chronic myeloid leukemia progression. The Journal of Experimental Medicine, 212(10), 1551-1569.

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