Maximal CPET was performed in all subjects and controls following a graded, symptom-limited cycle ergometry protocol (Godfrey, et al 1971 (link)). Subjects underwent testing at least 2 weeks after any vaso-occlusive pain episode requiring hospitalization and at least 3 months after any packed red blood cell transfusion. We used an electronically braked VIAsprint 150p Ergometric Bicycle (Carefusion Corp., San Diego, CA) with paediatric configurations and breath-by-breath gas exchange data was collected using a Vmax Encore 29C metabolic cart (CareFusion Corp.). Initial workload and workload increments were based on subject height, and workload increments occurred by continuous ramping at 1-min intervals until volitional exhaustion. Exercise testing was completed when subjects and controls could no longer maintain a cadence of 60 revolutions per minute after maximal effort, defined as having achieved a respiratory exchange ratio (RER) > 1.1. We calculated peak oxygen consumption (VO2) from 20-s averages of data points as the highest weight-adjusted value achieved in the last minute of exercise prior to recovery.
Vmax encore 29c
Manufactured by BD
Sourced in United States
The Vmax Encore 29C is a laboratory equipment designed for cell culture applications. It is a compact incubator that provides a controlled environment for cell growth and maintenance. The core function of the Vmax Encore 29C is to regulate temperature, humidity, and CO2 levels to create an optimal environment for cell culture.
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Lab products found in correlation
5 protocols using vmax encore 29c
1
Maximal CPET Protocol for Sickle Cell
2
Maximal Oxygen Consumption Testing Protocol
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Participants performed a maximal oxygen consumption exercise test on an electromagnetically controlled cycle ergometer (Ergometrics 800, Sensormedics, Yorba Linda, CA) to determine their peak oxygen consumption (VO2 peak). Oxygen uptake during the test was measured via an open-circuit sampling system (Vmax Encore 29C, Carefusion, Yorba Linda, CA), and the highest level of VO2 obtained for 1 min was defined as VO2 peak. Respiratory gas exchange was measured by having the participant wear a facemask that was connected to the Encore 29C system. The load on the bike was increased incrementally so that power output increased 25W per minute. The exercise test was performed until the participant was no longer able to maintain a cycling cadence above 40 revolutions per minute.
3
Pulmonary Function Measurement Protocol
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Forced expiratory volume in 1-s (FEV1), forced vital capacity (FVC) and the FEV1/FVC ratio were determined with participants seated, utilizing recommended techniques (Miller et al. 2005 (link)) and automated equipment (Vmax Encore 29C; CareFusion, Yorba Linda, CA, USA).
4
Spirometry and SVC Maneuvers Protocol
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5
Pulmonary Function Measurement Techniques
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Spirometry and plethysmography were performed with participants seated using automated equipment (Vmax EncoreTM 29C, CareFusion, Yorba Linda, CA, United States; Medisoft Body Box 5500®, Medisoft Belgium, Sorinnes, Belgium) and according to recommended techniques (Macintyre et al., 2005 (link); Miller et al., 2005a (link),b (link); Wanger et al., 2005 (link)). Measurements were referenced to predicted normal values (Briscoe and Dubois, 1958 (link); Burrows et al., 1961 (link); Crapo et al., 1981 (link); Hankinson et al., 1999 (link)).
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