A standardized set of Von Frey hairs (North Coast Medical, USA) that exert precise forces between 0.008 g and 300 g were applied perpendicularly to the skin, and the subject asked to report the sensation felt, if any. Children were asked to report when they first felt a sensation for MDT (this was repeated 3 times), and when they felt a sharp-prick like sensation for MPT (repeated 2 times at each site). Stimuli were applied at slightly different sites within a small area to avoid habituation.
Von frey hairs
Manufactured by North Coast Medical
Sourced in United States
Von Frey hairs are a set of calibrated monofilaments used to assess mechanical pain sensitivity. They are used to apply a standardized amount of force to the skin to evaluate somatosensory function.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using von frey hairs
1
Quantifying Sensory Perception in Children
2
Measuring Mechanical Hyperalgesia in Mice
3
Mechanical Hyperalgesia Assessment in Rodents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The animals were tested for mechanical hyperalgesia using the von Frey hairs (North Coast Medical USA) before injection and on postoperative days 3, 7, 14, 28, 42, and 56 (n = 8). Animals were habituated to the wire mesh bottom cages for 30 min each day for three days before the test. A serious of von Frey hairs in ascending order of force (1.0 g, 1.4 g, 2.0 g, 4.0 g, 6.0 g, 8.0 g, 10.0 g, 15.0 g, and 26 g) were applied to the plantar surface of the ipsilateral hind paws for 6 s. If withdrawal response was not induced, the next level of von Frey hair was applied until at least 5 withdrawal occurred in 10 stimuli (50% positive). The minimal force required to elicit 50% positive response was recorded as the paw withdrawal threshold.
4
Assessing Mechanical Nociception in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Von Frey hairs test was conducted to assess the mechanical nociception of mice (Zhu et al. 2010 ; Ma et al. 2015 ). Ten stimuli were made with each of a series of Von Frey hairs (North Coast Medical Inc, Morgan Hill, CA) comprising the first 11 monofilaments (0.008, 0.02, 0.04, 0.070, 0.16, 0.40, 0.60, 1.0, 1.4, 2.0, and 4.0 g). The test was performed as previously reported by Bourquin et al. (2006 ). The test was started with filament 0.008 g and the next stiffer monofilament was applied in turns. The positive response was determined by paw withdrawal occurring twice in the 10 applications. The monofilament that first evoked a positive response was defined as the threshold and no further monofilaments were applied. We measured the number of positive withdrawal responses in ascending order for each monofilament of the series. Relative frequency of paw withdrawal, the ratio of positive responses to the total 10 times, was calculated as another index to assess the mechanical sensitivity.
5
Quantifying Pain Sensitivity in Animals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pain sensitivity testing was performed before and after surgery or drug administration. The animals were habituated for 15 min in separate transparent Plexiglas chambers before tests. Mechanical sensitivity was tested with the up-down method using a set of von Frey hairs (0.4–25 g, North Coast medical) as described earlier.29 . Briefly, each von Frey hair application kept a 7 s on the sural nerve innervation area in the hindpaw. A quick withdrawal or licking paw indicates a positive response. The decrease in paw withdrawal thresholds (PWTs) indicates mechanical allodynia. Heat hypersensitivity was tested using Plantar Test System (7370, UgoBasile) described previously.30 (link) Briefly, a radiant heat from a light source under a transparent glass floor was applied at the hindpaw plantar surface alternately with 5 min intervals between each tests. Three measurements of paw withdrawal latencies (PWLs) were averaged.
6
Facial Mechanical Sensitivity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mechanical sensitivity of the facial skin was assessed by the use of von Frey hairs (North Coast Medical Inc., Morgan Hill, CA, USA) with a group of SCC-158 injected animals and a control groups as in a previous study.3 ) The von Frey filaments were applied to the forehead (ophthalmic nerve area), whisker-pad (maxillary nerve area), and submandibular (mandibular nerve area) skin areas. The frequency of nociceptive response (head withdrawal or vocalization) was counted from 5 trials. The nociceptive threshold was defined as the minimum pressure needed to evoke nociceptive responses in at least 60% of the trials.
7
Mechanical Sensitivity Threshold Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mechanical stimulus threshold was measured with von Frey hairs (North Coast Medical Inc., CA, USA). The animals were placed in wire mesh bottom cages and allowed to acclimatize for 30 min before examination. The plantar surface of the animal’s hind paw was stimulated with von Frey filament. Following application of calibrated von Frey filaments (0.4–15 g) with sufficient force to cause buckling of the filament, the 50% withdrawal threshold was determined according to the up-down method of Chaplan et al.17 (link) in an ascending order of force until a paw withdrawal response were elicited. Each von Frey hair was applied to the paw for 8 s or until a response occurred. Each rat was alternately tested three times with a 5-min intertrial interval.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!