Recombinant human bone morphogenetic protein 7

Poly(D, L-lactide-co-glycolide) 50:50 Resomer^® RG 503 (PLGA) (MW - 35 kDa) was purchased from Boehringer Ingelheim (Germany). Tetronic^® 1107 (T1107, HBL - 24 MW - 15 kDa), poly(vinyl alcohol) (PVA) (MW 31–50 kDa) heparin sodium salt grade IA from porcine intestinal mucosa, soybean lecithin, and cotton seed oil were obtained from Sigma Aldrich (Spain). Recombinant human bone morphogenetic protein 7 (BMP7) (pI 8.1 MW - 28.8 kDa), and the kit for its determination were purchased from PeproTech (UK) and the Abnova corporation (Taiwan), respectively. All other solvents and chemicals used were of high grade purity.

Boron doped silicon with different resistivity, 0.01–0.02 and 10–20 Ω cm, was purchased from Si Materials (Germany); wafer diameter was 100.0 ± 0.5 mm and thickness of 525 ± 25 µm (pI = 2–3.5). Fluoric acid (HF) (48%) was purchased from Riedel de Haën (Germany) and ethanol (96%) from Panreac (Barcelona, Spain). Synthetic air (N₂ with 21% of O₂) was provided from AbelloLinde S.A. (Barcelona, Spain). Avidin-peroxidase conjugate, α-chymotrypsinogen A (aCT) from bovine pancreas (pI = 9.5; Mw = 25.7 kDa), and 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) were obtained from Sigma Aldrich (Madrid, Spain). Recombinant human Bone Morphogenetic Protein-7 (BMP7) (pI = 8.1; Mw = 28.8 kDa), polyclonal antibody rabbit anti-human BMP7, and biotinylated polyclonal antibody rabbit anti-human BMP7 were purchased from PeproTech (London, UK). All other solvents and chemicals used were high-grade purity.