Dynamic light scattering (DLS) and zeta potential measurements were conducted using a Zetasizer Nano ZS (Malvern). Scanning electron microscopy (SEM) was conducted using a Zeiss Supra 25 Field Emission scanning electron microscope. SEM samples were prepared by gold sputtering and critical point drying. For AFM measurements, nanoparticles were observed on a freshly cleaved mica surface using an Asylum MFP 3D Bio AFM with an Olympus AC240TS probe. For transmission electron microscopy (TEM), a carbon-coated copper TEM grid (Ted Pella) was used with a JEOL 1200 EX transmission electron microscope operated at 80 kV.
Mfp 3d bio afm
Manufactured by Olympus
The MFP 3D Bio AFM is a high-resolution atomic force microscope designed for biological and life science applications. It provides researchers with the ability to image and analyze the topography and physical properties of samples at the nanoscale level. The instrument utilizes advanced scanning probe technology to capture detailed information about the surface structure and characteristics of biological samples, such as cells, proteins, and other biomolecules.
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2 protocols using mfp 3d bio afm
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Nanoparticle Characterization via Multimodal Analysis
2
Nanoparticle Characterization via Multimodal Analysis
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Dynamic light scattering (DLS) and zeta potential measurements were conducted using a Zetasizer Nano ZS (Malvern). Scanning electron microscopy (SEM) was conducted using a Zeiss Supra 25 Field Emission scanning electron microscope. SEM samples were prepared by gold sputtering and critical point drying. For AFM measurements, nanoparticles were observed on a freshly cleaved mica surface using an Asylum MFP 3D Bio AFM with an Olympus AC240TS probe. For transmission electron microscopy (TEM), a carbon-coated copper TEM grid (Ted Pella) was used with a JEOL 1200 EX transmission electron microscope operated at 80 kV.
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