Nod b10

Manufactured by Jackson ImmunoResearch

Sourced in United States

NOD.B10 is a mouse strain developed for research purposes. It is a non-obese diabetic background strain that carries the B10 major histocompatibility complex. The core function of this strain is to serve as a research model for the study of autoimmune disorders.

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Lab products found in correlation

Bl 10 mice, by Jackson ImmunoResearch (1 mentions) B6.129p2 sjl myd88tm1 1defr j mice, by Jackson ImmunoResearch (1 mentions) B6.129p2 sjl myd88tm1defr j, by Jackson ImmunoResearch (1 mentions)

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NOD.B10.H2b mice (7 citations)

4 protocols using nod b10

Murine Models of MyD88-Deficiency

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Age-matched male and female NOD.B10 and age-matched female BL/10 mice were used (Jackson Laboratory, Bar Harbor ME, USA). NOD.B10^Myd88+/− and NOD.B10^Myd88−/− mice were previously described (9 (link)). B6.129P2(SJL)-Myd88^tm1.1Defr/J mice were purchased from Jackson Laboratories. We generated BL/10 mice that are deficient in Myd88 by crossing BL/10 mice with Myd88^−/− mice (B6.129P2(SJL)-Myd88^tm1.1Defr/J). We then bred 10 successive generations of heterozygotes back to BL/10 mice to generate Myd88-deficient mice that are fully BL/10 (BL/10^Myd88−/−). Animals were bred and maintained at the University at Buffalo and housed in identical conditions. All animals used in this study were at least 26 weeks of age, the time that NOD.B10 females normally develop clinical disease (22 (link), 23 (link)). Mice were cared for and handled in accordance with the institutional animal care and use committee (IACUC) and US NIH guidelines.

Kiripolsky J., Romano R.A., Kasperek E.M., Yu G, & Kramer J.M. (2020). Activation of Myd88-Dependent TLRs Mediates Local and Systemic Inflammation in a Mouse Model of Primary Sjögren's Syndrome. Frontiers in Immunology, 10, 2963.

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Breeding and Maintaining NOD.B10 and BL/10 Mice

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NOD.B10 and C57BL10/SnJ (BL/10) mice were purchased from Jackson Laboratories. Animals were bred and maintained at the University at Buffalo. Females were used in all experiments. All animals were cared for and handled in accordance with NIH and IACUC guidelines.

Kiripolsky J., Shen L., Liang Y., Li A., Suresh L., Lian Y., Li Q.Z., Gaile D.P, & Kramer J.M. (2017). Systemic manifestations of primary Sjögren’s syndrome in the NOD.B10Sn-H2b/J mouse model. Clinical immunology (Orlando, Fla.), 183, 225-232.

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Generation and Validation of pSS Myd88 KO Mice

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BL/10 (stock# 000666) and NOD.B10 (stock# 002591) mice are available from Jackson Laboratories. Generation and validation of pSS conditional knockout mice that lack Myd88 in the hematopoietic compartment, referred to as NOD.B10^Myd88Δ, were described previously (25 (link)). Briefly, we first generated NOD.B10 mice that expressed Cre recombinase under the control of the Vav promoter (B6-Tg(vav1-icre)A2Kio/J) (Jackson Labs stock #008610) (29 (link), 30 (link)). We then bred Myd88 floxed animals (B6.129P2(SJL)-Myd88^tm1Defr/J) (Jackson Labs stock # 008888) to the NOD.B10 strain (Jackson Labs stock #002591) (31 (link)) to generate NOD.B10^Myd88fl/fl mice. Animals were backcrossed to the NOD.B10 strain for at least 6 generations and were verified to be fully congenic using a speed congenics approach (Jackson Laboratories). We then bred NOD.B10^Cre-Vav animals to the NOD.B10^Myd88fl/fl strain and the resultant progeny that expressed the Cre transgene under the control of the Vav promoter were designated as NOD.B10^Myd88Δ. Littermates that did not express the Cre transgene (NOD.B10^Myd88fl/fl) were employed as controls (25 (link)).
All animals used were females that were at least 26 weeks of age, the time at which the animals develop clinical disease (26 (link), 27 (link)). All animal experiments were carried out in accordance with IACUC and NIH guidelines.

Kiripolsky J., Kasperek E.M., Zhu C., Li Q.Z., Wang J., Yu G, & Kramer J.M. (2021). Immune-Intrinsic Myd88 Directs the Production of Antibodies With Specificity for Extracellular Matrix Components in Primary Sjögren’s Syndrome. Frontiers in Immunology, 12, 692216.

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Genetically Engineered Murine Model

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All animal experiments and procedures were performed in accordance with the State University of New York at Buffalo (University at Buffalo) Institutional Animal Care and Use Committee (IACUC) regulations. All procedures were approved by University at Buffalo IACUC. NOD.B10 and C57BL/10SnJ (BL/10) control mice were purchased from Jackson Laboratories (stock numbers #002591 and 000666, respectively). Female mice were used in all experiments.

Horeth E., Oyelakin A., Song E.A., Che M., Bard J., Min S., Kiripolsky J., Kramer J.M., Sinha S, & Romano R.A. (2021). Transcriptomic and Single-Cell Analysis Reveals Regulatory Networks and Cellular Heterogeneity in Mouse Primary Sjögren’s Syndrome Salivary Glands. Frontiers in Immunology, 12, 729040.

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