High sensitivity dna analysis kit

Manufactured by Agilent Technologies

Sourced in United States, Germany

The High Sensitivity DNA Analysis Kit is a laboratory equipment product designed for the analysis of DNA samples with high sensitivity. The kit provides the necessary reagents and protocols to perform accurate and reproducible DNA quantification and qualification across a wide range of sample types and concentrations.

Automatically generated - may contain errors

Lab products found in correlation

Qubit dsdna hs assay kit, by Thermo Fisher Scientific (15 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (13 mentions) Kapa library quantification kit, by Roche (10 mentions) Qubit 2.0 fluorometer, by Thermo Fisher Scientific (10 mentions) Nextseq 500, by Illumina (8 mentions) Hiseq 4000, by Illumina (8 mentions) Ampure xp bead, by Beckman Coulter (7 mentions) 2100 bioanalyzer, by Agilent Technologies (7 mentions) Qubit fluorometer, by Thermo Fisher Scientific (6 mentions) Trizol, by Thermo Fisher Scientific (6 mentions) Miseq platform, by Illumina (4 mentions) Hiseq 2500, by Illumina (4 mentions) Nextera xt dna library preparation kit, by Illumina (3 mentions) Qubit dsdna br assay kit, by Thermo Fisher Scientific (3 mentions) Bioanalyzer, by Agilent Technologies (3 mentions) Nextera xt dna sample preparation kit, by Illumina (3 mentions) Nebnext ultra rna library prep kit, by Illumina (3 mentions) Rneasy micro kit, by Qiagen (3 mentions) Miseq reagent kit v3, by Illumina (2 mentions) Nextera dna sample preparation kit, by Illumina (2 mentions)

Close spelling variants of this product

2100 Bioanalyzer High Sensitivity DNA Analysis Kit Agilent 2100 Bioanalyzer high sensitivity DNA analysis kit Agilent High Sensitivity DNA Analysis Kit DNF-488 High Sensitivity genomic DNA Analysis Kit High Sensitivity Genomic DNA Analysis Kit Bioanalyzer High Sensitivity DNA Analysis Kit

61 protocols using high sensitivity dna analysis kit

Shotgun Metagenomic Sequencing of Soil Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

For each of the duplicate soil samples a shotgun library was created. Shotgun
library preparation and metagenome sequencing was performed at IMGM Laboratories
GmbH (Martinsried, Germany). The shotgun library was prepared using the
Nextera^® XT Sample Preparation technology
(Illumina, San Diego, CA, USA). The libraries were size selected using
Agencourt^® AMPure^® XP
beads (Beckman Coulter, Pasadena, CA, USA) with a bead to DNA ratio of 0.6 to 1
(v/v). Quality and purity of the libraries has been analyzed with the High
Sensitivity DNA Analysis Kit (Agilent Technologies, Santa Clara, CA, USA) on a
2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Prior to library
normalization the libraries were quantified using the Quant-iT™
PicoGreen^® dsDNA assay kit (Invitrogen, Eugen,
OR, USA). Sequencing was performed on an Illumina
Miseq^® sequencing system (Illumina, San Diego,
CA, USA) with the MiSeq Reagent Kit v3 (Illumina, San Diego, CA, USA) resulting
in a read length of 2 × 300 bp.
Signal processing, de-multiplexing and trimming of adapter sequences were
performed using the MiSeq^® Reporter Software v.
2.3.32 (Illumina, San Diego, CA, USA).

Weigold P., El-Hadidi M., Ruecker A., Huson D.H., Scholten T., Jochmann M., Kappler A, & Behrens S. (2016). A metagenomic-based survey of microbial (de)halogenation potential in a German forest soil. Scientific Reports, 6, 28958.

+ Open protocol

+ Expand

Shotgun Metagenomic Analysis of Gut Microbiome

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was extracted from the ileum and colon faecal matter using the QIAamp DNA Stool Mini Kit (Qiagen). Colon and ileum DNA were combined and samples were pooled on a group basis for shotgun metagenomic analysis using the Ion Torrent PGM^™ platform. Pooled DNA (100 ng) was fragmented and barcoded using the NEBNext® Fast DNA Fragmentation and Library Prep Set for Ion Torrent^™ (NEB Inc, UK) and IonXpress Barcode Adapters kit (ThermoFisher Scientific) respectively. The quality and quantity of all barcoded DNA libraries were analysed using the High Sensitivity DNA analysis kit (Agilent, UK) on the 2100 Bioanalyzer Instrument and Qubit Fluorometer (ThermoFisher Scientific). Samples were prepared using the Ion PGM^™ Hi-Q^™ View OT2 and Ion PGM^™ Hi-Q^™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316^™ Chip kit (ThermoFisher Scientific). Data were analysed using MG-RAST and the number of reads per phylum, class, order, family or genera of species of interest were normalized against all bacteria present. Sequencing runs can be accessed using MG-RAST IDs mgl675297, mgl675300, mgl675312, mgl675291, mgl675309, mgl675318, mgl675306, mgl675294, mgl675321, mgl675315, mgl675285, mgl675288, mgl675324, mgl675303.

Crowe J., Lumb F.E., Doonan J., Broussard M., Tarafdar A., Pineda M.A., Landabaso C., Mulvey L., Hoskisson P.A., Babayan S.A., Selman C., Harnett W, & Harnett M.M. (2020). The parasitic worm product ES-62 promotes health- and life-span in a high calorie diet-accelerated mouse model of ageing. PLoS Pathogens, 16(3), e1008391.

+ Open protocol

+ Expand

Library Preparation for Genomic DNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

The purified genomic DNA was quantified using Qubit dsDNA HS Assay Kit (Life Technologies, USA) and normalized to 50 ng. Library was prepared using Nextera DNA Sample Preparation Kit (Illumina, USA) following the manufacturer’s protocols. Size estimation of the library was performed on a 2100 Bioanalyzer using High Sensitivity DNA Analysis Kit (Agilent Technologies) and quantified with Qubit 2.0 Fluorometer (Life Technologies, USA).

Yong H.S., Song S.L., Eamsobhana P., Goh S.Y., Lim P.E., Chow W.L., Chan K.G, & Abrahams-Sandi E. (2015). Mitochondrial Genome Supports Sibling Species of Angiostrongylus costaricensis (Nematoda: Angiostrongylidae). PLoS ONE, 10(7), e0134581.

+ Open protocol

+ Expand

Single-cell RNA-seq library preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cells were processed using a modified SMART-Seq2 protocol as previously described^{44 (link)}. Briefly, RNAClean XP beads (Agencourt) were used for RNA lysate cleanup, followed by reverse transcription using Maxima Reverse Transcriptase (Life Technologies), whole transcription amplification (WTA) with KAPA HotStart HIFI 2X ReadyMix (Kapa Biosystems) for 21 cycles and purification using AMPure XP beads (Agencourt). WTA products were quantified with Qubit dsDNA HS Assay Kit (ThermoFisher), visualized with high sensitivity DNA Analysis Kit (Agilent) and libraries were constructed using Nextera XT DNA Library Preparation Kit (Illumina). Population and no-cell controls were processed with the same methods as singe cells. Libraries were sequenced on an Illumina NextSeq 500.

Montoro D.T., Haber A.L., Biton M., Vinarsky V., Lin B., Birket S., Yuan F., Chen S., Leung H.M., Villoria J., Rogel N., Burgin G., Tsankov A., Waghray A., Slyper M., Waldmann J., Nguyen L., Dionne D., Rozenblatt-Rosen O., Tata P.R., Mou H., Shivaraju M., Bihler H., Mense M., Tearney G.J., Rowe S.M., Engelhardt J.F., Regev A, & Rajagopal J. (2018). A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature, 560(7718), 319-324.

+ Open protocol

+ Expand

mRNA Sequencing Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Messenger RNA (mRNA) was enriched with a NEBNext Poly (A) mRNA Magnetic Isolation Module (NEB, E7490, Ipswich, MA). Using the enriched mRNA as template, cDNA libraries were constructed using the NEBNext Ultra RNA Library Prep Kit Illumina (NEB, E7530, Ipswich, MA) and NEBNext Multiplex Oligos for Illumina (Index Primer 1–12) (NEB, E7600, Ipswich, MA) following the manufacturer’s instructions. To verify the quality, DNA concentration and product size of the cDNA libraries, a Qubit 2.0 Fluorometer (Life Technologies, Q32866), Qubit dsDNA BR assay kit (Life Technologies, Q32850), High Sensitivity DNA Analysis Kit (Agilent, 5067–4626) and Bioanalyzer were used. cDNA libraries were sequenced on an Illumina MiSeq Platform employing a 150 base pair single-end NGS setting. Data from MiSeq sequencing runs were uploaded and stored in BaseSpace (https://basespace.illumina.com) for data analysis.

Ballesteros C., Tritten L., O’Neill M., Burkman E., Zaky W.I., Xia J., Moorhead A., Williams S.A, & Geary T.G. (2016). The Effect of In Vitro Cultivation on the Transcriptome of Adult Brugia malayi. PLoS Neglected Tropical Diseases, 10(1), e0004311.

+ Open protocol

+ Expand

Efficient ChIP-seq Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sequencing libraries were prepared using ThruPLEX-FD kit (Rubicon Genomics). This kit reduces the assay time and the risk of contamination by using a single tube and eliminating intermediate purification steps. The process involved template preparation, library synthesis, and library amplification. Adaptor-based PCR amplification (98 °C for 20 s, 72 °C for 50 s for each cycle) was used during library amplification. We used 11 cycles for input DNA, 12~13 cycles for ChIP DNA from 10000 cells, and 14~17 cycles for ChIP DNA from 1000 or fewer cells. The libraries were purified using Ampure XP beads (A63880, Beckman Coulter). Library fragment size was determined using high sensitivity DNA analysis kit (5067-4626, Agilent) on an Agilent 2200 TapeStation. KAPA library quantification kit (KK4809, Kapa Biosystems) was used to determine effective library concentrations. The final concentrations of libraries submitted for sequencing were ~2 nM. The libraries were sequenced on an Illumina HiSeq 2500 with single-end 50 nt read. Typically 15–20 million reads were generated per library.

Cao Z., Chen C., He B., Tan K, & Lu C. (2015). A microfluidic device for epigenomic profiling using 100 cells. Nature methods, 12(10), 959-962.

+ Open protocol

+ Expand

Transcriptome Profiling by RNA-Seq

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted as previously reported [43 (link)]. Sample quality and quantity were checked with a Bioanalyzer and RNA 6000 Nano Kit (Agilent Technologies), and a Qubit with the RNA BR Assay Kit (Invitrogen). Sequence libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) and manufacturer’s instructions. Library quality and quantity were checked with a Bioanalyzer and the High Sensitivity DNA Analysis Kit (Agilent Technologies) and a Qubit with the ds DNA HS Assay Kit (Invitrogen). Samples were sequenced on a HiSeq1500 (Illumina) using 50 bp single-end sequencing in rapid-run mode. The dataset is available at the NCBI’s Gene Expression Omnibus under the accession code GSE101312.

de Bekker C., Will I., Hughes D.P., Brachmann A, & Merrow M. (2017). Daily rhythms and enrichment patterns in the transcriptome of the behavior-manipulating parasite Ophiocordyceps kimflemingiae. PLoS ONE, 12(11), e0187170.

+ Open protocol

+ Expand

RNA-seq Library Preparation and Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted from cells using Trizol using the manufacturer's instructions. RNA sequencing (RNA-seq) libraries were prepared from 4 μg of total RNA using the KAPA stranded mRNA-seq kit (Roche, KK8421) according to manufacturer's specifications. 100 nM KAPA-single index adapters (Roche, KK8700) were added to A-tailed cDNA, and libraries were amplified for 10 cycles. Finally, 1x library clean-up was performed using Agencourt AMPure XP beads (Beckman Coulter, A63881). Library fragment size was assessed using Agilent Bioanalyzer 2100 with the High Sensitivity DNA analysis kit (Agilent, 5067-4626) and concentration was determined using Qubit™ dsDNA HS Assay kit (Invitrogen, Q32854). Each library was diluted to 4 nM and pooled for sequencing on an Illumina Hiseq4000, aiming at 15–20 million SE50 reads per sample (19 million reads on average).

van der Veer B.K., Chen L., Custers C., Athanasouli P., Schroiff M., Cornelis R., Chui J.S., Finnell R.H., Lluis F, & Koh K.P. (2023). Dual functions of TET1 in germ layer lineage bifurcation distinguished by genomic context and dependence on 5-methylcytosine oxidation. Nucleic Acids Research, 51(11), 5469-5498.

+ Open protocol

+ Expand

Influenza A Virus Genomic Sequencing

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Complete genome has been amplified using the SuperScript III One-Step RT-PCR System and Platinum Taq High Fidelity (Invitrogen, Carlsbad, CA, USA) using one pair of primers complementary to the conserved elements of the influenza A virus promoter as previously described [24 (link)]. The sequencing library was prepared by using the Nex-tera DNA XT Sample preparation kit (Illumina, San Diego, CA, USA) and quantified by using the Qubit dsDNA High Sensitivity Kit (Invitrogen, Carlsbad, CA, USA). The High Sensitivity DNA Analysis Kit (Agilent Technologies, Alpharetta, GA, USA) was used to determine average fragment length. The indexed libraries were pooled in equimolar concentrations and sequenced in multiplex for 300 bp paired-end on Illumina MiSeq, according to the manufacturer’s instructions.

Rosone F., Bonfante F., Sala M.G., Maniero S., Cersini A., Ricci I., Garofalo L., Caciolo D., Denisi A., Napolitan A., Parente M., Zecchin B., Terregino C, & Scicluna M.T. (2023). Seroconversion of a Swine Herd in a Free-Range Rural Multi-Species Farm against HPAI H5N1 2.3.4.4b Clade Virus. Microorganisms, 11(5), 1162.

+ Open protocol

+ Expand

Mitochondrial DNA library preparation and sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA library was prepared using the VariantPro™ Mitochondrion Panel Library Preparation Kit (LC Sciences). The presence of the desired fragments and the purity of the indexed libraries were analysed on Agilent 2100 Bioanalyzer (Agilent Technologies) using High-Sensitivity DNA Analysis Kit (Agilent Technologies). The concentrations of the libraries were measured using Qubit Fluorometer (Thermo Fisher Scientific). Equimolar amounts of the 45 indexed libraries were pooled to obtain a 4 nM library mixture. After denaturing, and further diluting, the final 10 pM of library mixture was loaded into Illumina cartridge. Sequencing was performed using the Illumina MiSeq Reagent v2 kit (500 cycles) on the Illumina MiSeq instrument following the manufacturer’s instructions (Illumina).

Németh K., Darvasi O., Likó I., Szücs N., Czirják S., Reiniger L., Szabó B., Kurucz P.A., Krokker L., Igaz P., Patócs A, & Butz H. (2019). Next-generation sequencing identifies novel mitochondrial variants in pituitary adenomas. Journal of Endocrinological Investigation, 42(8), 931-940.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!