Invivovue oct system

Eyes were dilated with 1% atropine, followed by 2.5% phenylephrine hydrochloride (Alcon, Fort Worth, TX), and then mice were anesthetized with ketamine (72 mg/kg)/xylazine (4 mg/kg). One drop of 2.5% hydroxypropyl methylcellulose (Gonak; Akorn, Lake Forest, IL) was applied to each eye before examination. Spectral domain optical coherence tomography (SD-OCT) was performed using the InVivoVue OCT system (Bioptigen, Inc., Durham, NC). Three lateral images (nasal, central, and temporal) were collected, starting 0.2 mm above the meridian crossing through the center of the optic nerve (ON), at the ON meridian, and 0.2 mm below the ON meridian. A corresponding box centered on the ON with eight measurement points separated by 0.2 mm from each other was created. Corresponding neural retinas from different treatments were compared at the same location (0.2 mm temporal to the ON) from the vitreous face of the ganglion cell layer to the apical face of the RPE across the retina.