P0 human MSCs (n = 6) were thawed and seeded into T-150 flasks (Falcon) with 500,000 cells each, and cultured for 10 days in a humidified 5% CO2 atmosphere at 37 °C. Media were changed every 2 days. The media used for expansion were DMEM-HG with 10% FCS and 1% penicillin/streptomycin. After 7 days, 90% confluence was reached in all cells cultured and the cells were trypsinised.
T 150 flasks
Manufactured by BD
The T-150 flasks are a type of laboratory equipment designed for cell culture applications. They provide a controlled environment for the growth and maintenance of cells.
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Lab products found in correlation
5 protocols using t 150 flasks
1
Expansion of Human Mesenchymal Stem Cells
2
Expansion of human mesenchymal stem cells
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P0 human MSCs (n = 6) were thawed and seeded into T-150 flasks (Falcon) with 250,000 cells each and cultured for 10 days in a humidified 5% CO2 atmosphere at 37 °C. Media was changed every 2 days. The expansion media used was HG-DMEM with 10% FCS and 1% Pen/Strep. After 5 days, 80–90% confluence was reached in all cell cultures, and cells were trypsinized.
3
Expansion of BM-hMSC for Osteogenesis
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Before osteogenic differentiation, P1 BM-hMSC of 12 donors was defrosted, and 250,000 cells were seeded in gelatine-coated T150 flasks (Falcon) and cultured for 10 days in a humidified 5% CO2 atmosphere at 37 °C. Expansion media DMEM low glucose (DMEM LG) with 10% FCS and 1% Penicillin/Streptomycin were changed every second day. At a confluence of 80–90%, which was reached after approximately 5 days, cells were trypsinized and used for differentiation.
4
Expansion of Human Mesenchymal Stem Cells
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P0 hMSCs (n = 6) were thawed and seeded into T-150 flasks (Falcon) with a density of cells/cm2 and then cultured in a humidified atmosphere (5% CO2, 37 °C). Medium was changed every 2 days. Expansion medium was DMEM-HG with 10% FCS and 1% penicillin/streptomycin. After 90% confluence was reached in a cell cultures, the cells were trypsinised.
5
Expansion of Mesenchymal Stem Cells
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One vial of each donor (n = 6; MSC donor 1 to 6) was thawed and subsequently seeded into T-150 flasks (Falcon) with 30 ml of the expansion medium (EXP, medium B) at a density of 3.3 × 103/cm2. Cells were split when reaching 80% to 90% of confluence. Replating density was 3.3 × 103/cm2 in T-150 cell culture flasks. Cells were cultured for ten days until passage 3 (P3) to obtain a sufficient number of cells with media change every second day at 37°C and 5% CO2 (link). Finally, at 90% of confluence, cells were trypsinized (Trypsin/EDTA; Biochrom AG) and prepared for scaffold seeding.
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