Jsm 7600f scanning electron microscope

Cell measurements (n = 50) were made using the 40×/0.65 objective and an Olympus BX51 compound microscope. Four samples were examined in a JEOL JSM-7600F scanning electron microscope (SEM) at a working distance of 15 to 21 mm and a voltage of 1.2 to 5.0 kV after a preliminary wash in distilled water, followed by dehydration in a series of ethanol solutions of increasing concentration (30, 50, 70, 90 and 100%), air drying on 0.5″ aluminum mounts and sputter coating with gold–palladium using a Polaron SC7640 High Resolution Sputter Coater (Quorum Technologies, Newhaven, UK).

The 50–5000 nm aminated, carboxylated and NF particles were imaged using a scanning electron microscope to determine their sizes in the absence of an aqueous dispersion media. Round glass coverslips 12 mm diameter (Assistent, Germany) were cleaned with ethanol and attached to cylindrical aluminum mounts (Ted Pella Inc., Redding, California, USA) with silver paint (SPI Supplies, West Chester, Pennsylvania, USA). Using a glass pipette, a drop of the sterilized particles was deposited on each mounted coverslip, placed in a covered container, and allowed to air-dry at RT. Mounted dried samples were coated with a conductive layer of carbon in a high-vacuum evaporative coater (Cressington 208c, Ted Pella Inc., Redding, California, USA). Images were obtained with a JEOL JSM-7600F scanning electron microscope (JEOL USA Inc., Peabody, Massachusetts) operating at 2 kV.

Scanning electron microscopy (SEM) imaging was performed on some of the allantoic and amniotic fluid samples that had greatest DNA concentrations. The SEM imaging was carried out by the NDSU Electron Microscopy Center core facility. Allantoic and amniotic fluids (4 ml) were centrifuged for 10 min at 14,000 × g to produce a pellet, then the pellet was resuspended in deionized water. This process was repeated twice to remove buffer salts. Pelleted material was then applied to round glass coverslips affixed to aluminum mounts with silver paint (SPI Supplies, West Chester, PA, United States), air dried, and sputter coated with carbon (Cressington 208c, Ted Pella Inc., Redding, CA, United States). Images were obtained using a JEOL JSM-7600F scanning electron microscope at an accelerating voltage of 2 kV or a JEOL JSM-6490LV scanning electron microscopy at an accelerating voltage of 15 kV (JEOL USA Inc., Peabody, MA, United States).

After immersion in PBS, the PMMA beads were fractured using osteotomes. The gross appearance was observed to determine whether the sutures were completely hydrolyzed. The fracture surfaces were studied using a JSM-7600 F scanning electron microscope (SEM)(JEOL, Japan). An energy dispersive X-ray spectroscopy (EDS) was also used to visualize and analyze the surface features. The energy of the electron beam was 10 kV. To ensure high quality images, the samples were sputter-coated with platinum using a JFC 1600 auto-fine coater (JEOL, Japan).