P‐ERK (clone 197G2, Cell Signaling Technology, Danvers, MA, USA, #4377s, diluted 1:50). AffiniPure F(ab')2 fragment Donkey‐anti‐Rabbit IgG, conjugated to APC (#711‐136‐152, 1:50) or PE (#711‐116‐152, 1:50, Jackson ImmunoResearch, West Grove, PA, USA). Cleaved‐caspase 3‐Pacific Blue (clone D3E9, Cell Signaling Technology #8788s, diluted 1:100).
Affinipure f ab 2 fragment donkey anti rabbit igg
Manufactured by Jackson ImmunoResearch
Sourced in United States
AffiniPure F(ab')2 fragment Donkey‐anti‐Rabbit IgG is a laboratory reagent used to detect and quantify rabbit immunoglobulin (IgG) in various immunoassays and research applications. It is composed of the F(ab')2 fragment of donkey-derived antibodies that are specifically reactive with the Fc region of rabbit IgG.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved caspase 3 pacific blue clone d3e9, by Cell Signaling Technology (2 mentions)
Rabbit anti p akt, by Cell Signaling Technology (1 mentions)
Prolong diamond anti fade mount, by Thermo Fisher Scientific (1 mentions)
Pierce endogenous peroxidase suppressor, by Thermo Fisher Scientific (1 mentions)
3 protocols using affinipure f ab 2 fragment donkey anti rabbit igg
1
Phosphorylated ERK and Cleaved Caspase-3 Detection
2
Multiplexed Flow Cytometry Assay
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Anti-phosphorylated-S6 -PE (clone D57.2.2E, #5316 s, diluted 1:200), anti-phosphorylated ERK-AF647 (clone 137F5, #5376, diluted 1:50), cleaved caspase 3- pacific blue (clone D3E9, #8788, diluted 1:200), or anti-phosphorylated ERK (clone 197G2, #4377 s, diluted 1:50) all from Cell Signaling Technologies, Beverly, MA, USA). Unconjugated P-ERK was followed by AffiniPure F(ab')2 fragment Donkey-anti-Rabbit IgG, conjugated to APC (#711-136-152, diluted 1:50) or PE (#711-116-152, diluted 1:50, Jackson ImmunoResearch, West Grove, PA, USA), or cleaved PARP-AF647 (clone F21-852, #558710, diluted 1:100, BD Biosciences, San Jose, CA, USA)
3
Immunostaining with Monoclonal Antibodies
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Monoclonal antibodies, rabbit anti-pAkt and mouse anti-panAkt were purchased from Cell Signalling Technology. Pierce endogenous peroxidase suppressor was also obtained from Thermo Fisher Scientific (catalogue no. 35000). AffiniPure F(ab′)2 fragment donkey anti-rabbit IgG and peroxidase-conjugated AffiniPure F(ab′)2 fragment donkey anti-rabbit IgG were purchased from Jackson Immuno Research Laboratories. ATTO 488 NHS ester was purchased and was conjugated to the AffiniPure F(ab′)2 IgG as described in [4] (link). Prolong diamond anti-fade mount (catalogue no. P36970), bovine serum albumin (BSA) blocking reagent, amplification buffer and tyramide were all obtained from Life Technologies (catalogue nos. T20915COMPONENT - D, E and A).
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